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| Name | Guanidine Hydrochloride |
| Type | Chemical compound |
| Formula | CH5N3.HCl |
| Molecular Weight | 95.53 g/mol |
| Solubility | Highly soluble in water |
| Purity | ≥ 99% |
| Usage | Denaturant for proteins, RNA, and DNA |
| Storage Conditions | Store at room temperature |
| Appearance | White crystalline powder |
| CAS Number | 50-01-1 |
| EC Number | 200-002-3 |
| MDL Number | MFCD00013025 |
FAQ
What is Guanidine Hydrochloride and how is it used in molecular biology?
Guanidine Hydrochloride is a strong chaotropic agent that is commonly used in molecular biology for the denaturation of proteins and nucleic acids. It disrupts the structure of proteins by breaking hydrogen bonds and weakening hydrophobic interactions, allowing for easy separation of components in a sample.
Why is Guanidine Hydrochloride preferred over other denaturing agents in molecular biology experiments?
Guanidine Hydrochloride is preferred over other denaturing agents in molecular biology experiments because of its high denaturation efficiency, low viscosity, and ability to solubilize proteins efficiently. It is also less harsh on proteins compared to other denaturing agents like urea, making it a popular choice for protein purification and refolding experiments.
How is Guanidine Hydrochloride commonly used in protein purification processes?
Guanidine Hydrochloride is commonly used in protein purification processes as a denaturing agent to unfold proteins and expose hydrophobic regions. This allows for efficient separation of proteins from other cellular components, making it easier to isolate and purify the protein of interest. Guanidine Hydrochloride can also be used in refolding experiments to help renature denatured proteins back to their native conformation.
Can Guanidine Hydrochloride be used in nucleic acid extraction procedures?
Yes, Guanidine Hydrochloride can be used in nucleic acid extraction procedures to denature proteins that may interfere with the isolation of nucleic acids. By denaturing proteins with Guanidine Hydrochloride, nucleic acids can be effectively separated from proteins, lipids, and other cellular components, allowing for clean and pure nucleic acid samples to be obtained.
Are there any safety precautions that should be taken when working with Guanidine Hydrochloride in the laboratory?
When working with Guanidine Hydrochloride in the laboratory, it is important to wear appropriate personal protective equipment, including gloves, lab coat, and safety goggles. Guanidine Hydrochloride should be handled in a fume hood to minimize exposure to fumes and aerosols. It is also important to wash hands thoroughly after handling Guanidine Hydrochloride and avoid contact with skin and eyes.
Guanidine Hydrochloride is a strong chaotropic agent that is commonly used in molecular biology for the denaturation of proteins and nucleic acids. It disrupts the structure of proteins by breaking hydrogen bonds and weakening hydrophobic interactions, allowing for easy separation of components in a sample.
Why is Guanidine Hydrochloride preferred over other denaturing agents in molecular biology experiments?
Guanidine Hydrochloride is preferred over other denaturing agents in molecular biology experiments because of its high denaturation efficiency, low viscosity, and ability to solubilize proteins efficiently. It is also less harsh on proteins compared to other denaturing agents like urea, making it a popular choice for protein purification and refolding experiments.
How is Guanidine Hydrochloride commonly used in protein purification processes?
Guanidine Hydrochloride is commonly used in protein purification processes as a denaturing agent to unfold proteins and expose hydrophobic regions. This allows for efficient separation of proteins from other cellular components, making it easier to isolate and purify the protein of interest. Guanidine Hydrochloride can also be used in refolding experiments to help renature denatured proteins back to their native conformation.
Can Guanidine Hydrochloride be used in nucleic acid extraction procedures?
Yes, Guanidine Hydrochloride can be used in nucleic acid extraction procedures to denature proteins that may interfere with the isolation of nucleic acids. By denaturing proteins with Guanidine Hydrochloride, nucleic acids can be effectively separated from proteins, lipids, and other cellular components, allowing for clean and pure nucleic acid samples to be obtained.
Are there any safety precautions that should be taken when working with Guanidine Hydrochloride in the laboratory?
When working with Guanidine Hydrochloride in the laboratory, it is important to wear appropriate personal protective equipment, including gloves, lab coat, and safety goggles. Guanidine Hydrochloride should be handled in a fume hood to minimize exposure to fumes and aerosols. It is also important to wash hands thoroughly after handling Guanidine Hydrochloride and avoid contact with skin and eyes.
