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| Name | Guanidine Thiocyanate 6 mol/L (6 M in 0.1 M Tris; pH 7.5) |
| Category | Molecular Biology Product |
| Concentration | 6 mol/L |
| Buffer | 0.1 M Tris |
| PH | 7.5 |
| Attribute1 | Denaturant for nucleic acids |
| Attribute2 | Used in RNA extraction |
| Attribute3 | Disrupts protein-nucleic acid interactions |
| Attribute4 | Effective in inactivating RNases |
| Attribute5 | Stable under normal conditions |
| Attribute6 | High purity grade |
| Attribute7 | Store at room temperature |
| Attribute8 | Compatible with various downstream applications |
| Attribute9 | Quality tested for consistency |
| Attribute10 | Manufactured under ISO certified conditions |
FAQ
What is Guanidine Thiocyanate and how is it used in molecular biology?
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology for its ability to denature proteins and disrupt hydrogen bonding in nucleic acids. It is often used in nucleic acid extraction protocols to lyse cells and release DNA and RNA for downstream applications such as PCR, sequencing, and cloning.
How does the concentration of Guanidine Thiocyanate affect its performance in molecular biology applications?
The concentration of Guanidine Thiocyanate can significantly affect its performance in molecular biology applications. Higher concentrations of Guanidine Thiocyanate can increase the efficiency of nucleic acid extraction by promoting denaturation of proteins and nucleic acids, while lower concentrations may not be as effective in disrupting cellular structures.
What are the advantages of using Guanidine Thiocyanate in nucleic acid extraction protocols?
One of the main advantages of using Guanidine Thiocyanate in nucleic acid extraction protocols is its chaotropic properties, which help to disrupt cell membranes and release DNA and RNA. Additionally, Guanidine Thiocyanate is stable at room temperature, making it convenient for laboratory use.
How does the pH of the solution containing Guanidine Thiocyanate affect its performance in nucleic acid extraction?
The pH of the solution containing Guanidine Thiocyanate is important for optimal performance in nucleic acid extraction protocols. A pH of 7.5 is commonly used as it is conducive to denaturation of proteins and nucleic acids. Deviations from this pH can affect the efficiency of nucleic acid extraction and should be carefully monitored.
In what type of samples is Guanidine Thiocyanate 6 mol/L most commonly used for nucleic acid extraction?
Guanidine Thiocyanate 6 mol/L is commonly used in nucleic acid extraction from a variety of sample types, including cultured cells, tissues, blood, and other bodily fluids. Its high chaotropic properties make it effective in extracting DNA and RNA from a wide range of sources in molecular biology applications.
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology for its ability to denature proteins and disrupt hydrogen bonding in nucleic acids. It is often used in nucleic acid extraction protocols to lyse cells and release DNA and RNA for downstream applications such as PCR, sequencing, and cloning.
How does the concentration of Guanidine Thiocyanate affect its performance in molecular biology applications?
The concentration of Guanidine Thiocyanate can significantly affect its performance in molecular biology applications. Higher concentrations of Guanidine Thiocyanate can increase the efficiency of nucleic acid extraction by promoting denaturation of proteins and nucleic acids, while lower concentrations may not be as effective in disrupting cellular structures.
What are the advantages of using Guanidine Thiocyanate in nucleic acid extraction protocols?
One of the main advantages of using Guanidine Thiocyanate in nucleic acid extraction protocols is its chaotropic properties, which help to disrupt cell membranes and release DNA and RNA. Additionally, Guanidine Thiocyanate is stable at room temperature, making it convenient for laboratory use.
How does the pH of the solution containing Guanidine Thiocyanate affect its performance in nucleic acid extraction?
The pH of the solution containing Guanidine Thiocyanate is important for optimal performance in nucleic acid extraction protocols. A pH of 7.5 is commonly used as it is conducive to denaturation of proteins and nucleic acids. Deviations from this pH can affect the efficiency of nucleic acid extraction and should be carefully monitored.
In what type of samples is Guanidine Thiocyanate 6 mol/L most commonly used for nucleic acid extraction?
Guanidine Thiocyanate 6 mol/L is commonly used in nucleic acid extraction from a variety of sample types, including cultured cells, tissues, blood, and other bodily fluids. Its high chaotropic properties make it effective in extracting DNA and RNA from a wide range of sources in molecular biology applications.
