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| Name | Guanidine Thiocyanate |
| Category | Molecular Biology |
| Cas Number | 593-84-0 |
| Molecular Formula | CH5N3SCN |
| Appearance | White crystalline solid |
| Solubility | Soluble in water, ethanol, and acetone |
| Purity | ≥ 99% |
| Storage Condition | Store at room temperature |
| Usage | Commonly used in nucleic acid extraction protocols |
| Applications | RNA/DNA extraction, protein denaturation |
| Molar Mass | 118.15 g/mol |
| Melting Point | 120-122°C |
| Boiling Point | Decomposes above 160°C |
| Odor | Characteristic odor |
| Handling Precautions | Wear gloves and eye protection when handling |
| Supplier | Multiple suppliers available worldwide |
FAQ
What is Guanidine Thiocyanate and how is it used in molecular biology studies?
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology studies to lyse cells and denature proteins. It disrupts hydrogen bonding and hydrophobic interactions, allowing for the isolation and purification of nucleic acids such as DNA and RNA from biological samples.
What are the advantages of using Guanidine Thiocyanate in molecular biology research?
One of the main advantages of using Guanidine Thiocyanate is its ability to effectively denature proteins and disrupt cellular membranes, allowing for the extraction of high-quality nucleic acids. Additionally, it can be used at a wide range of concentrations to optimize the lysis and purification process for specific samples.
How does Guanidine Thiocyanate compare to other chaotropic agents commonly used in molecular biology?
Guanidine Thiocyanate is often preferred over other chaotropic agents like guanidine hydrochloride or urea due to its higher chaotropic activity and ability to inhibit RNase activity. It also has a higher solubility in water, making it easier to prepare solutions for use in molecular biology protocols.
What are some common applications of Guanidine Thiocyanate in molecular biology research?
Guanidine Thiocyanate is commonly used in protocols for RNA isolation, DNA extraction, and protein purification. It is particularly useful in studies involving gene expression analysis, PCR amplification, and sequencing, where high-quality nucleic acids are essential for accurate results.
Are there any important safety considerations to keep in mind when working with Guanidine Thiocyanate?
Guanidine Thiocyanate is a hazardous chemical that can cause skin and respiratory irritation, so it is important to handle it with care in a well-ventilated area. It should be stored in a tightly sealed container away from heat and light to prevent degradation. Additionally, personal protective equipment such as gloves, goggles, and lab coats should be worn when working with Guanidine Thiocyanate to avoid any potential health risks.
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology studies to lyse cells and denature proteins. It disrupts hydrogen bonding and hydrophobic interactions, allowing for the isolation and purification of nucleic acids such as DNA and RNA from biological samples.
What are the advantages of using Guanidine Thiocyanate in molecular biology research?
One of the main advantages of using Guanidine Thiocyanate is its ability to effectively denature proteins and disrupt cellular membranes, allowing for the extraction of high-quality nucleic acids. Additionally, it can be used at a wide range of concentrations to optimize the lysis and purification process for specific samples.
How does Guanidine Thiocyanate compare to other chaotropic agents commonly used in molecular biology?
Guanidine Thiocyanate is often preferred over other chaotropic agents like guanidine hydrochloride or urea due to its higher chaotropic activity and ability to inhibit RNase activity. It also has a higher solubility in water, making it easier to prepare solutions for use in molecular biology protocols.
What are some common applications of Guanidine Thiocyanate in molecular biology research?
Guanidine Thiocyanate is commonly used in protocols for RNA isolation, DNA extraction, and protein purification. It is particularly useful in studies involving gene expression analysis, PCR amplification, and sequencing, where high-quality nucleic acids are essential for accurate results.
Are there any important safety considerations to keep in mind when working with Guanidine Thiocyanate?
Guanidine Thiocyanate is a hazardous chemical that can cause skin and respiratory irritation, so it is important to handle it with care in a well-ventilated area. It should be stored in a tightly sealed container away from heat and light to prevent degradation. Additionally, personal protective equipment such as gloves, goggles, and lab coats should be worn when working with Guanidine Thiocyanate to avoid any potential health risks.
