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Product Name | SDS - Solution 10% |
Product Type | Molecular biology reagent |
Concentration | 10% |
Formulation | Sodium dodecyl sulfate solution |
Storage Condition | Store at room temperature |
Application | Used for denaturing nucleic acids and proteins |
Purity | High purity |
Stability | Stable for long term storage |
Compatibility | Compatible with common molecular biology techniques |
Packaging | Available in various volumes |
Solubility | Soluble in water |
Shelf Life | Long shelf life |
Hazard Warnings | Harmful if swallowed, avoid contact with eyes and skin, wear appropriate protective gear |
Shipping Restrictions | May have restrictions for international shipping |
Manufacturer | Various manufacturers available in the market |
FAQ
What is SDS - Solution 10% and how does it benefit molecular biology research?
SDS - Solution 10% is a powerful denaturing agent commonly used in molecular biology research. It helps to disrupt the structure of proteins and other biological molecules, making them more accessible for analysis and manipulation. In particular, SDS - Solution 10% is useful for solubilizing proteins in samples for techniques such as gel electrophoresis and western blotting.
How is SDS - Solution 10% used in protein extraction and sample preparation?
SDS - Solution 10% is often used in protein extraction protocols to lyse cells and solubilize proteins. By adding SDS - Solution 10% to a cell lysate, researchers can disrupt cell membranes and denature proteins, allowing for their separation and analysis. Additionally, SDS - Solution 10% is commonly used in sample preparation for techniques such as western blotting, where it helps to denature proteins and coat them with a negative charge for separation by electrophoresis.
What are the advantages of using SDS - Solution 10% in molecular biology experiments?
One of the primary advantages of using SDS - Solution 10% in molecular biology experiments is its ability to denature proteins and other biomolecules, making them more amenable to analysis. Additionally, SDS - Solution 10% helps to solubilize proteins, enabling their separation based on size and charge in techniques such as gel electrophoresis. Moreover, SDS - Solution 10% is generally easy to use and compatible with a wide range of downstream applications, making it a versatile reagent for molecular biology research.
Can SDS - Solution 10% be used in conjunction with other reagents or techniques?
Yes, SDS - Solution 10% can be used in conjunction with other reagents and techniques commonly utilized in molecular biology research. For example, it is often used in combination with reducing agents and protease inhibitors to ensure proper denaturation and stabilization of proteins. Additionally, SDS - Solution 10% can be used in conjunction with techniques such as western blotting, protein purification, and protein quantification assays to enhance the accuracy and reliability of experimental results.
How should SDS - Solution 10% be stored and handled to maintain its effectiveness?
SDS - Solution 10% should be stored at room temperature and protected from light to maintain its stability and effectiveness. It is important to avoid repeated freeze-thaw cycles, as this can degrade the reagent and compromise its performance. When handling SDS - Solution 10%, it is crucial to wear appropriate personal protective equipment, such as gloves and eye protection, to prevent skin and eye irritation. Additionally, care should be taken to use the reagent in a well-ventilated area to prevent inhalation of fumes.
SDS - Solution 10% is a powerful denaturing agent commonly used in molecular biology research. It helps to disrupt the structure of proteins and other biological molecules, making them more accessible for analysis and manipulation. In particular, SDS - Solution 10% is useful for solubilizing proteins in samples for techniques such as gel electrophoresis and western blotting.
How is SDS - Solution 10% used in protein extraction and sample preparation?
SDS - Solution 10% is often used in protein extraction protocols to lyse cells and solubilize proteins. By adding SDS - Solution 10% to a cell lysate, researchers can disrupt cell membranes and denature proteins, allowing for their separation and analysis. Additionally, SDS - Solution 10% is commonly used in sample preparation for techniques such as western blotting, where it helps to denature proteins and coat them with a negative charge for separation by electrophoresis.
What are the advantages of using SDS - Solution 10% in molecular biology experiments?
One of the primary advantages of using SDS - Solution 10% in molecular biology experiments is its ability to denature proteins and other biomolecules, making them more amenable to analysis. Additionally, SDS - Solution 10% helps to solubilize proteins, enabling their separation based on size and charge in techniques such as gel electrophoresis. Moreover, SDS - Solution 10% is generally easy to use and compatible with a wide range of downstream applications, making it a versatile reagent for molecular biology research.
Can SDS - Solution 10% be used in conjunction with other reagents or techniques?
Yes, SDS - Solution 10% can be used in conjunction with other reagents and techniques commonly utilized in molecular biology research. For example, it is often used in combination with reducing agents and protease inhibitors to ensure proper denaturation and stabilization of proteins. Additionally, SDS - Solution 10% can be used in conjunction with techniques such as western blotting, protein purification, and protein quantification assays to enhance the accuracy and reliability of experimental results.
How should SDS - Solution 10% be stored and handled to maintain its effectiveness?
SDS - Solution 10% should be stored at room temperature and protected from light to maintain its stability and effectiveness. It is important to avoid repeated freeze-thaw cycles, as this can degrade the reagent and compromise its performance. When handling SDS - Solution 10%, it is crucial to wear appropriate personal protective equipment, such as gloves and eye protection, to prevent skin and eye irritation. Additionally, care should be taken to use the reagent in a well-ventilated area to prevent inhalation of fumes.