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| Name | SSC buffer (20X) |
| Application | Molecular biology |
| Concentration | 20X |
| Buffer Components | Saline, Sodium citrate |
| PH Range | 7.0 - 7.6 |
| Storage Conditions | Store at room temperature |
| Shelf Life | 1 year |
| Usage | Hybridization procedures, especially for nucleic acid hybridization |
| Compatibility | DNA and RNA samples |
| Preparation | Dilute with water to desired concentration before use |
| Volume | Various sizes available |
| Color | Clear |
| Format | Liquid |
| Safety Information | Avoid contact with skin and eyes |
| Shipping Information | Shipped at room temperature |
FAQ
What is the SSC buffer (20X) used for in molecular biology applications?
The SSC buffer (20X) is commonly used in molecular biology applications for various purposes, including nucleic acid hybridization, blotting techniques, and other procedures that require stringent washing conditions.
What are the key benefits of using the SSC buffer (20X) in molecular biology experiments?
The SSC buffer (20X) offers a high salt concentration, which helps to facilitate the hybridization of nucleic acids by providing optimal stringency. It also helps to reduce nonspecific binding of nucleic acids, resulting in cleaner and more accurate results.
How do I prepare a working solution of SSC buffer from the 20X concentrate?
To prepare a working solution of SSC buffer from the 20X concentrate, simply dilute the 20X stock solution with the appropriate amount of distilled water to achieve the desired final concentration. The specific dilution factor will depend on the particular application and the required salt concentration.
Can the SSC buffer (20X) be used for both DNA and RNA applications in molecular biology?
Yes, the SSC buffer (20X) can be used for both DNA and RNA applications in molecular biology. It is commonly used in nucleic acid hybridization techniques, such as Southern and Northern blotting, to provide the necessary stringency for accurate and specific hybridization of DNA or RNA probes.
Are there any storage conditions that I should follow for the SSC buffer (20X) to maintain its stability and effectiveness?
To ensure the stability and effectiveness of the SSC buffer (20X), it is recommended to store the concentrate at room temperature and protect it from light. Avoid repeated freeze-thaw cycles, as this may affect the performance of the buffer. Additionally, always check the expiration date on the label before use to ensure optimal results.
The SSC buffer (20X) is commonly used in molecular biology applications for various purposes, including nucleic acid hybridization, blotting techniques, and other procedures that require stringent washing conditions.
What are the key benefits of using the SSC buffer (20X) in molecular biology experiments?
The SSC buffer (20X) offers a high salt concentration, which helps to facilitate the hybridization of nucleic acids by providing optimal stringency. It also helps to reduce nonspecific binding of nucleic acids, resulting in cleaner and more accurate results.
How do I prepare a working solution of SSC buffer from the 20X concentrate?
To prepare a working solution of SSC buffer from the 20X concentrate, simply dilute the 20X stock solution with the appropriate amount of distilled water to achieve the desired final concentration. The specific dilution factor will depend on the particular application and the required salt concentration.
Can the SSC buffer (20X) be used for both DNA and RNA applications in molecular biology?
Yes, the SSC buffer (20X) can be used for both DNA and RNA applications in molecular biology. It is commonly used in nucleic acid hybridization techniques, such as Southern and Northern blotting, to provide the necessary stringency for accurate and specific hybridization of DNA or RNA probes.
Are there any storage conditions that I should follow for the SSC buffer (20X) to maintain its stability and effectiveness?
To ensure the stability and effectiveness of the SSC buffer (20X), it is recommended to store the concentrate at room temperature and protect it from light. Avoid repeated freeze-thaw cycles, as this may affect the performance of the buffer. Additionally, always check the expiration date on the label before use to ensure optimal results.
