TAE buffer (10X) for molecular biology

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Name TAE buffer (10X)
Use Molecular biology applications
PH 8.3
Composition Tris, acetic acid, EDTA
Concentration 10X
Storage Conditions Store at room temperature
Compatibility Suitable for agarose gel electrophoresis
Applications DNA and RNA agarose gel electrophoresis
Stability Stable at room temperature
Quantity 1 L
Buffering Capacity Maintains stable pH during electrophoresis
Shipping Conditions Shipped at ambient temperature
Manufacturer Various
Shelf Life 12 months
Preparation Dilute 10X stock to desired concentration before use
Safety Information Avoid contact with skin and eyes
Availability Widely available from multiple suppliers
Price Range Varies depending on supplier
Quality Control Tested for performance and consistency
FAQ
What is TAE buffer (10X) and how is it used in molecular biology?
TAE buffer (10X) is a common buffer solution used in molecular biology for various applications such as agarose gel electrophoresis, DNA and RNA agarose gel electrophoresis, and DNA and RNA fragment separation. It serves as a running buffer in electrophoresis and provides the necessary ions and pH conditions for optimal separation of nucleic acid molecules.

What are the components of TAE buffer (10X) and how do they contribute to its function?
TAE buffer (10X) typically contains Tris base, acetic acid, and EDTA. Tris base helps maintain a stable pH environment for the electrophoresis process, acetic acid provides conductivity for the buffer solution, and EDTA helps to chelate divalent metal ions that can interfere with the electrophoresis process.

How is TAE buffer (10X) prepared and what is the recommended storage method?
To prepare TAE buffer (10X), the individual components are dissolved in distilled water and the pH is adjusted to the desired level using a strong acid or base. The buffer solution is then sterilized by filtration or autoclaving before use. It is recommended to store the prepared TAE buffer at room temperature away from direct sunlight to maintain its stability and performance.

What are the advantages of using TAE buffer (10X) over other buffer solutions in molecular biology experiments?
TAE buffer (10X) is preferred by many researchers for its ability to provide consistent and reliable results in DNA and RNA electrophoresis. It has a higher buffering capacity compared to other buffer solutions, allowing for longer electrophoresis runs without significant pH changes. Additionally, TAE buffer is cost-effective and easy to prepare in the laboratory.

What are some common troubleshooting tips when using TAE buffer (10X) in molecular biology experiments?
If encountering issues with TAE buffer (10X) during electrophoresis, it is important to check the pH of the buffer solution to ensure it is within the optimal range for nucleic acid separation. Contaminants in the buffer can also affect the quality of results, so always use freshly prepared or properly stored TAE buffer. In case of poor DNA or RNA resolution, adjusting the agarose concentration or the voltage used in electrophoresis may help improve the separation of nucleic acid fragments.
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