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| Product Name | TBE buffer (5X) |
| Applications | used in molecular biology for agarose gel electrophoresis of nucleic acids |
| Composition | Tris, boric acid, EDTA |
| PH | approximately 8.3 |
| Concentration | 5X concentrated |
| Storage Condition | store at room temperature |
| Shipping Condition | shipped at room temperature |
| Color | clear colorless solution |
| Volume | 500 ml |
| Manufacturing Company | varies by brand |
FAQ
What is TBE buffer and how is it used in molecular biology?
TBE buffer is a commonly used buffer solution in molecular biology for running agarose gel electrophoresis. It is used to create an optimal environment for separating DNA fragments based on their size.
What are the advantages of using TBE buffer?
TBE buffer has several advantages, including its ability to provide stable pH levels during electrophoresis, its compatibility with many types of DNA samples, and its excellent buffering capacity which helps maintain DNA integrity.
How is TBE buffer prepared for use in experiments?
TBE buffer is typically prepared by mixing Tris base, boric acid, and EDTA in distilled water to achieve the desired concentration. The solution is then adjusted to the appropriate pH level using concentrated acid or base.
What are some common applications of TBE buffer in molecular biology research?
TBE buffer is commonly used for DNA fragment analysis, RNA electrophoresis, nucleic acid gel staining, and protein electrophoresis. It is an essential component in many molecular biology experiments due to its buffering properties and compatibility with nucleic acids.
Are there any special storage requirements for TBE buffer?
TBE buffer should be stored at room temperature and protected from light to prevent degradation. It should also be kept tightly sealed to prevent evaporation and contamination.
TBE buffer is a commonly used buffer solution in molecular biology for running agarose gel electrophoresis. It is used to create an optimal environment for separating DNA fragments based on their size.
What are the advantages of using TBE buffer?
TBE buffer has several advantages, including its ability to provide stable pH levels during electrophoresis, its compatibility with many types of DNA samples, and its excellent buffering capacity which helps maintain DNA integrity.
How is TBE buffer prepared for use in experiments?
TBE buffer is typically prepared by mixing Tris base, boric acid, and EDTA in distilled water to achieve the desired concentration. The solution is then adjusted to the appropriate pH level using concentrated acid or base.
What are some common applications of TBE buffer in molecular biology research?
TBE buffer is commonly used for DNA fragment analysis, RNA electrophoresis, nucleic acid gel staining, and protein electrophoresis. It is an essential component in many molecular biology experiments due to its buffering properties and compatibility with nucleic acids.
Are there any special storage requirements for TBE buffer?
TBE buffer should be stored at room temperature and protected from light to prevent degradation. It should also be kept tightly sealed to prevent evaporation and contamination.
