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| Name | TE buffer (1X) pH 8.0 |
| Used For | Molecular biology applications |
| PH Level | 8.0 |
| Concentration | 1X |
| Composition | Tris-Cl and EDTA |
| Storage Conditions | Store at room temperature |
| Appearance | Clear, colorless solution |
| Function | Used for DNA and RNA extraction, storage, and dilution |
| Stability | Stable at room temperature for long periods |
| Compatibility | Compatible with most molecular biology applications |
| Quality Control | Tested for pH and sterility |
| Sterility | Non-sterile, not suitable for cell culture |
| Manufacturer | Various molecular biology product manufacturers |
| Shipping Conditions | Shipped at room temperature |
| Safety Information | Avoid contact with skin and eyes, wear appropriate PPE |
| Hazards | May be harmful if ingested, inhaled, or absorbed through skin |
| Hazmat Shipping | Not classified as hazardous for shipping |
FAQ
What is TE buffer (1X) pH 8.0 and how is it used in molecular biology?
TE buffer (Tris-EDTA buffer) is a widely used buffer in molecular biology for various applications such as DNA and RNA extraction, storage, and manipulation. At a pH of 8.0, TE buffer is commonly used to stabilize nucleic acids and protect them from degradation.
What are the components of TE buffer (1X) pH 8.0?
TE buffer (1X) pH 8.0 is composed of two main components: Tris-HCl and EDTA. Tris-HCl provides the buffering capacity needed to maintain a stable pH, while EDTA chelates divalent cations such as Mg2+ that can degrade nucleic acids.
How is TE buffer (1X) pH 8.0 prepared for use in molecular biology experiments?
To prepare TE buffer (1X) pH 8.0, one would typically mix Tris-HCl and EDTA in the appropriate concentrations and adjust the pH to 8.0 using a strong acid or base. The buffer solution can then be sterilized by filtration or autoclaving before use.
What are the benefits of using TE buffer (1X) pH 8.0 in molecular biology experiments?
TE buffer (1X) pH 8.0 offers several advantages in molecular biology experiments. It helps stabilize nucleic acids, prevents enzymatic degradation, and maintains the integrity of DNA and RNA samples. Additionally, the buffer is compatible with a wide range of molecular biology techniques.
How should TE buffer (1X) pH 8.0 be stored to maintain its stability and effectiveness?
TE buffer (1X) pH 8.0 should be stored at room temperature or in a refrigerator when not in use to maintain its stability and effectiveness. It is important to avoid repeated freeze-thaw cycles as this can affect the quality of the buffer.
TE buffer (Tris-EDTA buffer) is a widely used buffer in molecular biology for various applications such as DNA and RNA extraction, storage, and manipulation. At a pH of 8.0, TE buffer is commonly used to stabilize nucleic acids and protect them from degradation.
What are the components of TE buffer (1X) pH 8.0?
TE buffer (1X) pH 8.0 is composed of two main components: Tris-HCl and EDTA. Tris-HCl provides the buffering capacity needed to maintain a stable pH, while EDTA chelates divalent cations such as Mg2+ that can degrade nucleic acids.
How is TE buffer (1X) pH 8.0 prepared for use in molecular biology experiments?
To prepare TE buffer (1X) pH 8.0, one would typically mix Tris-HCl and EDTA in the appropriate concentrations and adjust the pH to 8.0 using a strong acid or base. The buffer solution can then be sterilized by filtration or autoclaving before use.
What are the benefits of using TE buffer (1X) pH 8.0 in molecular biology experiments?
TE buffer (1X) pH 8.0 offers several advantages in molecular biology experiments. It helps stabilize nucleic acids, prevents enzymatic degradation, and maintains the integrity of DNA and RNA samples. Additionally, the buffer is compatible with a wide range of molecular biology techniques.
How should TE buffer (1X) pH 8.0 be stored to maintain its stability and effectiveness?
TE buffer (1X) pH 8.0 should be stored at room temperature or in a refrigerator when not in use to maintain its stability and effectiveness. It is important to avoid repeated freeze-thaw cycles as this can affect the quality of the buffer.
