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| Name | TE buffer (1X) pH 8.0 low EDTA |
| Type | Molecular biology product |
| PH | 8.0 |
| Concentration | 1X |
| Composition | Tris-HCl, EDTA |
| Purpose | Used in molecular biology for DNA and RNA experiments |
| Storage | Store at room temperature |
| Stability | Stable for at least 1 year when stored properly |
| Buffering Agent | Tris-HCl |
| Chelating Agent | EDTA |
| Color | Colorless |
| Odor | Odorless |
| Solubility | Soluble in water |
| Applications | DNA and RNA purification, PCR, DNA sequencing |
FAQ
What is TE buffer (1X) pH 8.0 low EDTA used for in molecular biology?
TE buffer (1X) pH 8.0 low EDTA is commonly used in molecular biology for DNA and RNA purification, storage, and manipulation. It is a commonly used solution for stabilizing nucleic acids in solution, protecting them from degradation by nucleases, and for maintaining the integrity of DNA and RNA samples during various molecular biology procedures.
What are the advantages of using TE buffer (1X) pH 8.0 low EDTA in molecular biology experiments?
One of the key advantages of using TE buffer (1X) pH 8.0 low EDTA is that it helps maintain the stability and integrity of nucleic acids, such as DNA and RNA, by providing a suitable environment for their storage and manipulation. The EDTA in the buffer helps to chelate divalent cations, such as magnesium and calcium, which can interfere with DNA and RNA stability. Additionally, the pH of the buffer is optimized for DNA and RNA stability, making it an ideal choice for various molecular biology applications.
How is TE buffer (1X) pH 8.0 low EDTA prepared and stored for use in molecular biology experiments?
TE buffer (1X) pH 8.0 low EDTA is typically prepared by diluting a concentrated stock solution to the desired working concentration of 1X. To prepare the buffer, the appropriate amount of stock solution is added to distilled water, taking care to mix the solution thoroughly to ensure homogeneity. The buffer can be stored at room temperature or at 4°C for short-term storage. It is important to protect the buffer from contaminants and ensure that it remains sterile for optimal performance in molecular biology experiments.
What are some common applications of TE buffer (1X) pH 8.0 low EDTA in molecular biology research?
TE buffer (1X) pH 8.0 low EDTA is commonly used in a wide range of molecular biology applications, including DNA and RNA extraction, purification, and storage. It is used to resuspend DNA pellets, dilute DNA samples for downstream analysis, and stabilize nucleic acids in solution for various molecular biology techniques, such as PCR, sequencing, and cloning. Additionally, TE buffer (1X) pH 8.0 low EDTA is used in gel electrophoresis for DNA and RNA sample loading and analysis, as well as in hybridization experiments for nucleic acid detection.
What are some tips for using TE buffer (1X) pH 8.0 low EDTA effectively in molecular biology experiments?
When using TE buffer (1X) pH 8.0 low EDTA in molecular biology experiments, it is important to handle the buffer with care to avoid contamination and degradation of nucleic acids. Be sure to use sterile techniques when preparing and handling the buffer to prevent introduction of exogenous nucleases. Additionally, be mindful of the pH and temperature of the buffer, as deviations from the optimal conditions can affect the stability of nucleic acids. Store the buffer properly and avoid repeated freeze-thaw cycles to maintain its efficacy in molecular biology applications.
TE buffer (1X) pH 8.0 low EDTA is commonly used in molecular biology for DNA and RNA purification, storage, and manipulation. It is a commonly used solution for stabilizing nucleic acids in solution, protecting them from degradation by nucleases, and for maintaining the integrity of DNA and RNA samples during various molecular biology procedures.
What are the advantages of using TE buffer (1X) pH 8.0 low EDTA in molecular biology experiments?
One of the key advantages of using TE buffer (1X) pH 8.0 low EDTA is that it helps maintain the stability and integrity of nucleic acids, such as DNA and RNA, by providing a suitable environment for their storage and manipulation. The EDTA in the buffer helps to chelate divalent cations, such as magnesium and calcium, which can interfere with DNA and RNA stability. Additionally, the pH of the buffer is optimized for DNA and RNA stability, making it an ideal choice for various molecular biology applications.
How is TE buffer (1X) pH 8.0 low EDTA prepared and stored for use in molecular biology experiments?
TE buffer (1X) pH 8.0 low EDTA is typically prepared by diluting a concentrated stock solution to the desired working concentration of 1X. To prepare the buffer, the appropriate amount of stock solution is added to distilled water, taking care to mix the solution thoroughly to ensure homogeneity. The buffer can be stored at room temperature or at 4°C for short-term storage. It is important to protect the buffer from contaminants and ensure that it remains sterile for optimal performance in molecular biology experiments.
What are some common applications of TE buffer (1X) pH 8.0 low EDTA in molecular biology research?
TE buffer (1X) pH 8.0 low EDTA is commonly used in a wide range of molecular biology applications, including DNA and RNA extraction, purification, and storage. It is used to resuspend DNA pellets, dilute DNA samples for downstream analysis, and stabilize nucleic acids in solution for various molecular biology techniques, such as PCR, sequencing, and cloning. Additionally, TE buffer (1X) pH 8.0 low EDTA is used in gel electrophoresis for DNA and RNA sample loading and analysis, as well as in hybridization experiments for nucleic acid detection.
What are some tips for using TE buffer (1X) pH 8.0 low EDTA effectively in molecular biology experiments?
When using TE buffer (1X) pH 8.0 low EDTA in molecular biology experiments, it is important to handle the buffer with care to avoid contamination and degradation of nucleic acids. Be sure to use sterile techniques when preparing and handling the buffer to prevent introduction of exogenous nucleases. Additionally, be mindful of the pH and temperature of the buffer, as deviations from the optimal conditions can affect the stability of nucleic acids. Store the buffer properly and avoid repeated freeze-thaw cycles to maintain its efficacy in molecular biology applications.
