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| Name | Tris Hydrochloride |
| Category | Molecular Biology |
| Form | Powder |
| Purity | ≥99% |
| Molecular Weight | 157.60 g/mol |
| Solubility | Soluble in water |
| Storage Temperature | Room temperature |
| Appearance | White crystalline powder |
| CAS Number | 1185-53-1 |
| Molecular Formula | C4H11NO3·HCl |
| Boiling Point | 219 °C |
| Melting Point | 171-175 °C |
| Density | 1.01 g/cm3 |
| Usage | Used in molecular biology applications such as buffer preparation and protein crystallization |
| Shelf Life | 2 years |
FAQ
What is Tris Hydrochloride and how is it used in molecular biology?
Tris Hydrochloride is a buffer commonly used in molecular biology applications. It helps maintain a stable pH level in various biological and chemical reactions. Tris Hydrochloride is typically used in DNA and RNA isolation, protein purification, enzyme assays, and other biochemical experiments.
What are the advantages of using Tris Hydrochloride in molecular biology experiments?
One of the main advantages of using Tris Hydrochloride is its ability to maintain a stable pH level in a solution. This is crucial for many biological reactions to occur efficiently. Tris Hydrochloride is also relatively inexpensive and easy to use, making it a popular choice for researchers in the field of molecular biology.
How should Tris Hydrochloride be stored to maintain its stability and effectiveness?
Tris Hydrochloride should be stored in a cool, dry place away from direct sunlight. It is important to keep the container tightly sealed to prevent contamination. Additionally, Tris Hydrochloride should be kept at the appropriate temperature recommended by the manufacturer to ensure its stability and effectiveness.
What are some common applications of Tris Hydrochloride in molecular biology research?
Tris Hydrochloride is commonly used as a buffer in various molecular biology techniques such as gel electrophoresis, PCR, western blotting, and DNA sequencing. It helps maintain a stable pH environment for these reactions to occur optimally. Tris Hydrochloride is also used in the preparation of cell culture media and various biochemical assays.
Are there any safety precautions that should be taken when working with Tris Hydrochloride in the laboratory?
When working with Tris Hydrochloride in the laboratory, it is important to wear appropriate personal protective equipment, such as gloves and lab coat, to prevent skin contact. In case of accidental ingestion or exposure, seek medical attention immediately. Tris Hydrochloride should be handled with care and disposed of properly according to the laboratory's waste management protocols.
Tris Hydrochloride is a buffer commonly used in molecular biology applications. It helps maintain a stable pH level in various biological and chemical reactions. Tris Hydrochloride is typically used in DNA and RNA isolation, protein purification, enzyme assays, and other biochemical experiments.
What are the advantages of using Tris Hydrochloride in molecular biology experiments?
One of the main advantages of using Tris Hydrochloride is its ability to maintain a stable pH level in a solution. This is crucial for many biological reactions to occur efficiently. Tris Hydrochloride is also relatively inexpensive and easy to use, making it a popular choice for researchers in the field of molecular biology.
How should Tris Hydrochloride be stored to maintain its stability and effectiveness?
Tris Hydrochloride should be stored in a cool, dry place away from direct sunlight. It is important to keep the container tightly sealed to prevent contamination. Additionally, Tris Hydrochloride should be kept at the appropriate temperature recommended by the manufacturer to ensure its stability and effectiveness.
What are some common applications of Tris Hydrochloride in molecular biology research?
Tris Hydrochloride is commonly used as a buffer in various molecular biology techniques such as gel electrophoresis, PCR, western blotting, and DNA sequencing. It helps maintain a stable pH environment for these reactions to occur optimally. Tris Hydrochloride is also used in the preparation of cell culture media and various biochemical assays.
Are there any safety precautions that should be taken when working with Tris Hydrochloride in the laboratory?
When working with Tris Hydrochloride in the laboratory, it is important to wear appropriate personal protective equipment, such as gloves and lab coat, to prevent skin contact. In case of accidental ingestion or exposure, seek medical attention immediately. Tris Hydrochloride should be handled with care and disposed of properly according to the laboratory's waste management protocols.
