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| Name | Tris buffer pH 8.0 (1 M) |
| Category | Molecular Biology |
| PH | 8 |
| Concentration | 1 M |
| Size | 1 L |
| Storage Condition | Store at room temperature |
| Composition | Tris base |
| Usage | Buffer solution for molecular biology experiments |
| Compatibility | Compatible with common laboratory reagents |
| Stability | Stable for long-term storage |
| Form | Liquid |
| Color | Colorless |
| Odor | Odorless |
| Solubility | Soluble in water |
| Purity | High purity |
| Manufacturing Method | Manufactured using high-quality materials |
| Expiration Date | 2 years from manufacturing date |
| Manufacturer | Various manufacturers |
FAQ
What is the pH of Tris buffer pH 8.0 (1 M) and why is it important for molecular
biology?
Tris buffer pH 8.0 (1 M) has a pH of 8.0, which is ideal for many molecular biology applications. This specific pH level helps to maintain a stable environment for various biological reactions, such as enzyme activity and nucleic acid stability. It is crucial for maintaining the proper pH balance in solutions used for molecular biology experiments.
How is Tris buffer pH 8.0 (1 M) prepared and stored?
Tris buffer pH 8.0 (1 M) can be prepared by dissolving Tris base in water and adjusting the pH to 8.0 with hydrochloric acid. It should be stored at room temperature away from direct sunlight and extreme temperatures to prevent degradation. It is recommended to aliquot the buffer into smaller volumes for ease of use and to avoid repeated freeze-thaw cycles.
What are the applications of Tris buffer pH 8.0 (1 M) in molecular biology?
Tris buffer pH 8.0 (1 M) is commonly used in molecular biology for various applications, including DNA and RNA isolation, polymerase chain reaction (PCR), protein electrophoresis, and western blotting. It provides a stable pH environment that helps maintain the integrity and functionality of biological molecules during experimental procedures.
How does Tris buffer pH 8.0 (1 M) compare to other types of buffers in molecular biology?
Tris buffer pH 8.0 (1 M) is preferred by many researchers for its stability and compatibility with a wide range of biological applications. It has a pH of 8.0, which is suitable for most biological reactions, and it can be easily prepared and adjusted to the desired pH level. Tris buffer is also less likely to interfere with downstream assays compared to other buffers, making it a popular choice in molecular biology research.
Can Tris buffer pH 8.0 (1 M) be used in combination with other buffers or reagents in molecular biology experiments?
Yes, Tris buffer pH 8.0 (1 M) can be used in combination with other buffers or reagents in molecular biology experiments. It is often used in conjunction with other components, such as EDTA, sodium chloride, or glycerol, to create customized buffers for specific applications. However, it is important to ensure compatibility and appropriate concentrations of all reagents to avoid any interference with experimental results.
Tris buffer pH 8.0 (1 M) has a pH of 8.0, which is ideal for many molecular biology applications. This specific pH level helps to maintain a stable environment for various biological reactions, such as enzyme activity and nucleic acid stability. It is crucial for maintaining the proper pH balance in solutions used for molecular biology experiments.
How is Tris buffer pH 8.0 (1 M) prepared and stored?
Tris buffer pH 8.0 (1 M) can be prepared by dissolving Tris base in water and adjusting the pH to 8.0 with hydrochloric acid. It should be stored at room temperature away from direct sunlight and extreme temperatures to prevent degradation. It is recommended to aliquot the buffer into smaller volumes for ease of use and to avoid repeated freeze-thaw cycles.
What are the applications of Tris buffer pH 8.0 (1 M) in molecular biology?
Tris buffer pH 8.0 (1 M) is commonly used in molecular biology for various applications, including DNA and RNA isolation, polymerase chain reaction (PCR), protein electrophoresis, and western blotting. It provides a stable pH environment that helps maintain the integrity and functionality of biological molecules during experimental procedures.
How does Tris buffer pH 8.0 (1 M) compare to other types of buffers in molecular biology?
Tris buffer pH 8.0 (1 M) is preferred by many researchers for its stability and compatibility with a wide range of biological applications. It has a pH of 8.0, which is suitable for most biological reactions, and it can be easily prepared and adjusted to the desired pH level. Tris buffer is also less likely to interfere with downstream assays compared to other buffers, making it a popular choice in molecular biology research.
Can Tris buffer pH 8.0 (1 M) be used in combination with other buffers or reagents in molecular biology experiments?
Yes, Tris buffer pH 8.0 (1 M) can be used in combination with other buffers or reagents in molecular biology experiments. It is often used in conjunction with other components, such as EDTA, sodium chloride, or glycerol, to create customized buffers for specific applications. However, it is important to ensure compatibility and appropriate concentrations of all reagents to avoid any interference with experimental results.
