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| Product Name | X-Gal for Molecular Biology |
| Supplier | Multiple |
| Usage | Substrate for beta-galactosidase in molecular biology applications |
| Appearance | White to off-white powder |
| Solubility | Soluble in DMF (Dimethylformamide) |
| Storage Conditions | Store at -20°C, protect from light |
| Molecular Weight | 408.3 g/mol |
| CAS Number | 7240-90-6 |
| Purity | ≥ 99% |
| Shelf Life | 2 years |
| Applications | Cloning, gene expression studies, and blue/white screening |
| Handling Precautions | Avoid inhalation, ingestion and contact with skin and eyes |
FAQ
What is X-Gal and how is it used in molecular biology studies?
X-Gal, or 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, is a synthetic chromogenic substrate used to detect the activity of beta-galactosidase in molecular biology studies.
What is the significance of using X-Gal in molecular biology experiments?
X-Gal is commonly used in molecular biology experiments as a substrate for beta-galactosidase to identify recombinant cells or organisms.
How is X-Gal incorporated into experimental protocols in molecular biology studies?
X-Gal is often included in growth media or reaction buffers to allow for the screening of beta-galactosidase activity in cells or organisms.
What are some common applications of X-Gal in molecular biology research?
X-Gal is frequently used in bacterial transformation assays, blue-white screening, and other genetic engineering techniques to identify successfully transformed cells.
How does X-Gal function in molecular biology experiments and what are the benefits of using this substrate?
X-Gal is cleaved by beta-galactosidase to produce a blue precipitate, allowing for easy visualization and identification of positive results in molecular biology experiments.
X-Gal, or 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, is a synthetic chromogenic substrate used to detect the activity of beta-galactosidase in molecular biology studies.
What is the significance of using X-Gal in molecular biology experiments?
X-Gal is commonly used in molecular biology experiments as a substrate for beta-galactosidase to identify recombinant cells or organisms.
How is X-Gal incorporated into experimental protocols in molecular biology studies?
X-Gal is often included in growth media or reaction buffers to allow for the screening of beta-galactosidase activity in cells or organisms.
What are some common applications of X-Gal in molecular biology research?
X-Gal is frequently used in bacterial transformation assays, blue-white screening, and other genetic engineering techniques to identify successfully transformed cells.
How does X-Gal function in molecular biology experiments and what are the benefits of using this substrate?
X-Gal is cleaved by beta-galactosidase to produce a blue precipitate, allowing for easy visualization and identification of positive results in molecular biology experiments.
