Back
| Name | ß-Mercaptoethanol |
| Category | Molecular Biology |
| Purity | ≥99% |
| Appearance | Colorless liquid |
| Odor | Characteristic odor |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.13 g/mol |
| Boiling Point | 99-100°C |
| Melting Point | -10°C |
| Solubility | Soluble in water, alcohol |
| Storage Conditions | Store at room temperature |
| Shelf Life | 2 years |
| Applications | Reducing agent, protein denaturant, solubilizing agent |
FAQ
What is ß-Mercaptoethanol and how is it used in molecular biology?
ß-Mercaptoethanol, also known as BME, is a reducing agent commonly used in molecular biology and biochemistry applications. It is used to break disulfide bonds in proteins, which helps to denature and unfold proteins for analysis or manipulation. BME is particularly useful in reducing agents and in protein purification procedures.
What are the benefits of using ß-Mercaptoethanol in molecular biology experiments?
ß-Mercaptoethanol offers several benefits in molecular biology experiments. It helps to break disulfide bonds in proteins, allowing for better denaturation and manipulation of proteins. It also helps to reduce protein aggregation, leading to cleaner and more accurate results. Additionally, BME is useful in preserving RNA integrity by breaking down RNAases that degrade RNA samples.
How should ß-Mercaptoethanol be stored and handled in the laboratory?
ß-Mercaptoethanol should be stored in a cool, dry place away from light and moisture to prevent degradation. It is important to handle BME with care as it is a hazardous chemical that can cause skin and respiratory irritation. Always use appropriate personal protective equipment, such as gloves and goggles, when handling BME in the laboratory.
Are there any precautions or safety measures that should be taken when working with ß-Mercaptoethanol?
When working with ß-Mercaptoethanol, it is important to follow proper safety precautions to minimize the risk of exposure. Avoid direct contact with skin, eyes, and mucous membranes, and use in a well-ventilated area to prevent inhalation of vapors. In case of skin or eye contact, rinse thoroughly with water. If swallowed, seek medical attention immediately.
In what types of molecular biology experiments is ß-Mercaptoethanol commonly used?
ß-Mercaptoethanol is commonly used in a variety of molecular biology experiments, including protein denaturation, reducing protein aggregation, and preserving RNA integrity. It is often added to lysis buffers for protein extraction, as well as to RNA isolation kits to prevent RNA degradation. BME is also used in Western blotting, SDS-PAGE, and other protein analysis techniques.
ß-Mercaptoethanol, also known as BME, is a reducing agent commonly used in molecular biology and biochemistry applications. It is used to break disulfide bonds in proteins, which helps to denature and unfold proteins for analysis or manipulation. BME is particularly useful in reducing agents and in protein purification procedures.
What are the benefits of using ß-Mercaptoethanol in molecular biology experiments?
ß-Mercaptoethanol offers several benefits in molecular biology experiments. It helps to break disulfide bonds in proteins, allowing for better denaturation and manipulation of proteins. It also helps to reduce protein aggregation, leading to cleaner and more accurate results. Additionally, BME is useful in preserving RNA integrity by breaking down RNAases that degrade RNA samples.
How should ß-Mercaptoethanol be stored and handled in the laboratory?
ß-Mercaptoethanol should be stored in a cool, dry place away from light and moisture to prevent degradation. It is important to handle BME with care as it is a hazardous chemical that can cause skin and respiratory irritation. Always use appropriate personal protective equipment, such as gloves and goggles, when handling BME in the laboratory.
Are there any precautions or safety measures that should be taken when working with ß-Mercaptoethanol?
When working with ß-Mercaptoethanol, it is important to follow proper safety precautions to minimize the risk of exposure. Avoid direct contact with skin, eyes, and mucous membranes, and use in a well-ventilated area to prevent inhalation of vapors. In case of skin or eye contact, rinse thoroughly with water. If swallowed, seek medical attention immediately.
In what types of molecular biology experiments is ß-Mercaptoethanol commonly used?
ß-Mercaptoethanol is commonly used in a variety of molecular biology experiments, including protein denaturation, reducing protein aggregation, and preserving RNA integrity. It is often added to lysis buffers for protein extraction, as well as to RNA isolation kits to prevent RNA degradation. BME is also used in Western blotting, SDS-PAGE, and other protein analysis techniques.
