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| Name | 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica |
| Cas Number | 74127-27-8 |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 g/mol |
| Appearance | Yellow to orange powder |
| Solubility | Soluble in DMSO, DMF |
| Storage Conditions | Store at -20°C in a dry, well-ventilated area |
| Purity | ≥98% |
| Usage | Substrate for detection of ß-galactosidase activity |
| Shelf Life | 2 years |
| Melting Point | 150-152°C |
| Boiling Point | N/A |
| Flash Point | N/A |
| Specific Gravity | N/A |
| Handling | Avoid inhalation, ingestion, and contact with skin and eyes |
FAQ
What is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica and what is its
application?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is a chemical compound that is commonly used in biochemical studies to detect the presence of β-D-galactosidase enzyme. This compound is specifically designed to interact with the enzyme and produce a yellow product when the enzyme cleaves the compound. This reaction is widely used as a convenient and rapid method to assess the activity of β-D-galactosidase in various biological samples.
How is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica used in research and experimentation?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is typically added to a reaction mixture containing the biological sample and the β-D-galactosidase enzyme. The reaction is then monitored for the formation of a yellow product, which indicates the presence and activity of the enzyme. This compound is commonly used in laboratory settings to study gene expression, cell signaling pathways, and enzyme kinetics.
What are the benefits of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in biochemical research?
One of the main advantages of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is its specificity towards β-D-galactosidase enzyme. This compound allows researchers to quickly and easily detect the enzymatic activity of β-D-galactosidase in biological samples. Additionally, the yellow product produced in the reaction provides a visual indicator of enzyme activity, making the assay simple and straightforward to interpret.
Can 2-Nitrophenyl-β-D-Galactopyranoside BioChemica be used in high-throughput screening applications?
Yes, 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is well-suited for high-throughput screening applications due to its rapid and sensitive detection of β-D-galactosidase activity. This compound can be easily incorporated into automated systems for quick and efficient analysis of large sample sets. Its compatibility with microplate readers and other robotic platforms makes it an ideal choice for screening programs aimed at identifying potential enzyme inhibitors or activators.
What storage and handling recommendations should be followed when using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica should be stored at -20°C in a dry, dark place to prevent degradation. The compound should be handled with care to avoid exposure to light and moisture, which can affect its stability. Prior to use, the compound should be allowed to reach room temperature to prevent condensation inside the container. It is important to follow the manufacturer's instructions for proper storage and handling of 2-Nitrophenyl-β-D-Galactopyranoside BioChemica to ensure reliable results in biochemical experiments.
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is a chemical compound that is commonly used in biochemical studies to detect the presence of β-D-galactosidase enzyme. This compound is specifically designed to interact with the enzyme and produce a yellow product when the enzyme cleaves the compound. This reaction is widely used as a convenient and rapid method to assess the activity of β-D-galactosidase in various biological samples.
How is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica used in research and experimentation?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is typically added to a reaction mixture containing the biological sample and the β-D-galactosidase enzyme. The reaction is then monitored for the formation of a yellow product, which indicates the presence and activity of the enzyme. This compound is commonly used in laboratory settings to study gene expression, cell signaling pathways, and enzyme kinetics.
What are the benefits of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in biochemical research?
One of the main advantages of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is its specificity towards β-D-galactosidase enzyme. This compound allows researchers to quickly and easily detect the enzymatic activity of β-D-galactosidase in biological samples. Additionally, the yellow product produced in the reaction provides a visual indicator of enzyme activity, making the assay simple and straightforward to interpret.
Can 2-Nitrophenyl-β-D-Galactopyranoside BioChemica be used in high-throughput screening applications?
Yes, 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is well-suited for high-throughput screening applications due to its rapid and sensitive detection of β-D-galactosidase activity. This compound can be easily incorporated into automated systems for quick and efficient analysis of large sample sets. Its compatibility with microplate readers and other robotic platforms makes it an ideal choice for screening programs aimed at identifying potential enzyme inhibitors or activators.
What storage and handling recommendations should be followed when using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica should be stored at -20°C in a dry, dark place to prevent degradation. The compound should be handled with care to avoid exposure to light and moisture, which can affect its stability. Prior to use, the compound should be allowed to reach room temperature to prevent condensation inside the container. It is important to follow the manufacturer's instructions for proper storage and handling of 2-Nitrophenyl-β-D-Galactopyranoside BioChemica to ensure reliable results in biochemical experiments.
