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| Product Name | 3,3',5,5'-Tetramethylbenzidine |
| CAS Number | 54827-17-7 |
| Molecular Formula | C16H20N2 |
| Molecular Weight | 240.34 g/mol |
| Appearance | White to off-white crystalline powder |
| Melting Point | 168-172°C |
| Solubility | Soluble in DMSO, ethanol, acetone |
| Storage | Store at room temperature |
| Stability | Stable under recommended storage conditions |
| Usage | Commonly used in ELISA and other biochemical assays |
| Purity | ≥98% |
| Boiling Point | 372.5°C |
FAQ
What is 3,3',5,5'-Tetramethylbenzidine (TMB)?
3,3',5,5'-Tetramethylbenzidine (TMB) is a commonly used chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical assays. It is widely used in biotechnology, clinical diagnostics, and research laboratories for detecting the presence of enzymes such as horseradish peroxidase.
How does 3,3',5,5'-Tetramethylbenzidine work in ELISA assays?
In ELISA assays, TMB is used as a substrate for horseradish peroxidase (HRP), an enzyme commonly used as a label to detect the presence of target analytes. When HRP catalyzes the oxidation of TMB in the presence of hydrogen peroxide, a blue-colored product is formed. The intensity of the color is directly proportional to the amount of HRP present in the sample, allowing for quantitative analysis of the target analyte.
What are the advantages of using 3,3',5,5'-Tetramethylbenzidine in biochemical assays?
TMB offers several advantages for use in biochemical assays, including high sensitivity, excellent stability, and a wide dynamic range for colorimetric detection. Its ease of use and compatibility with a variety of enzyme labels make it a popular choice for researchers and clinicians alike.
How should 3,3',5,5'-Tetramethylbenzidine be stored and handled in the laboratory?
TMB should be stored in a cool, dark place away from light and moisture to maintain its stability. It is important to handle TMB with care to avoid contact with skin or inhalation of fumes. Proper safety precautions, such as wearing gloves and using a fume hood, should be followed when working with TMB to ensure safe laboratory practices.
Can 3,3',5,5'-Tetramethylbenzidine be used in combination with other substrates or detection systems?
Yes, TMB can be used in combination with other substrates or detection systems to enhance the sensitivity or specificity of biochemical assays. Researchers often optimize their protocols by testing different substrates or detection systems to achieve the best results for their specific experimental conditions.
3,3',5,5'-Tetramethylbenzidine (TMB) is a commonly used chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) and other biochemical assays. It is widely used in biotechnology, clinical diagnostics, and research laboratories for detecting the presence of enzymes such as horseradish peroxidase.
How does 3,3',5,5'-Tetramethylbenzidine work in ELISA assays?
In ELISA assays, TMB is used as a substrate for horseradish peroxidase (HRP), an enzyme commonly used as a label to detect the presence of target analytes. When HRP catalyzes the oxidation of TMB in the presence of hydrogen peroxide, a blue-colored product is formed. The intensity of the color is directly proportional to the amount of HRP present in the sample, allowing for quantitative analysis of the target analyte.
What are the advantages of using 3,3',5,5'-Tetramethylbenzidine in biochemical assays?
TMB offers several advantages for use in biochemical assays, including high sensitivity, excellent stability, and a wide dynamic range for colorimetric detection. Its ease of use and compatibility with a variety of enzyme labels make it a popular choice for researchers and clinicians alike.
How should 3,3',5,5'-Tetramethylbenzidine be stored and handled in the laboratory?
TMB should be stored in a cool, dark place away from light and moisture to maintain its stability. It is important to handle TMB with care to avoid contact with skin or inhalation of fumes. Proper safety precautions, such as wearing gloves and using a fume hood, should be followed when working with TMB to ensure safe laboratory practices.
Can 3,3',5,5'-Tetramethylbenzidine be used in combination with other substrates or detection systems?
Yes, TMB can be used in combination with other substrates or detection systems to enhance the sensitivity or specificity of biochemical assays. Researchers often optimize their protocols by testing different substrates or detection systems to achieve the best results for their specific experimental conditions.
