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| Product Name | Acrylamide 2K - Solution (18%) |
| Usage | Denaturing DNA-PAGE |
| Concentration | 18% |
| Appearance | Clear liquid |
| Storage Conditions | Store at -20°C |
| Stability | Stable for at least 6 months |
| Solubility | Soluble in water |
| Handling Precautions | Avoid inhalation and skin contact |
| Hazards | Acute toxicity, skin and eye irritation |
| Purity | ≥ 99% |
| Packaging | Comes in amber glass bottle |
FAQ
What is Acrylamide 2K - Solution (18%) used for in denaturing DNA-PAGE?
Acrylamide 2K - Solution (18%) is commonly used in denaturing DNA-PAGE (polyacrylamide gel electrophoresis) to separate DNA fragments based on their size. The acrylamide solution helps to create a gel matrix in which the DNA molecules can migrate through when an electrical current is applied, allowing for the separation and analysis of DNA fragments.
How is Acrylamide 2K - Solution (18%) prepared for use in denaturing DNA-PAGE?
To prepare Acrylamide 2K - Solution (18%) for use in denaturing DNA-PAGE, the solution is typically mixed with a cross-linking agent such as N,N’-methylenebisacrylamide (Bis) and other components to create a gel mixture. This gel mixture is then poured into a mold and allowed to polymerize, forming a solid gel that is ready for the electrophoresis of DNA samples.
What are the advantages of using Acrylamide 2K - Solution (18%) for denaturing DNA-PAGE?
Acrylamide 2K - Solution (18%) offers several advantages for denaturing DNA-PAGE, including high resolution and sharp band separation, making it ideal for the analysis of DNA fragments with similar sizes. The 18% concentration of acrylamide in the solution allows for the separation of small DNA fragments with high accuracy, making it a popular choice for research and diagnostic applications.
Can Acrylamide 2K - Solution (18%) be used for other applications besides denaturing DNA-PAGE?
While Acrylamide 2K - Solution (18%) is primarily used for denaturing DNA-PAGE, it can also be utilized for other applications such as protein electrophoresis and other molecular biology techniques that require the separation of biomolecules based on their size. However, it is important to note that the concentration of acrylamide may need to be adjusted depending on the specific requirements of the experiment.
Is Acrylamide 2K - Solution (18%) safe to use in the laboratory setting?
Acrylamide 2K - Solution (18%) should be handled with care in the laboratory setting, as acrylamide is a known neurotoxin and possible carcinogen. It is important to take necessary precautions when working with acrylamide solutions, such as wearing appropriate personal protective equipment (PPE) and following proper safety protocols. Additionally, disposal of acrylamide waste should be done according to regulations to minimize environmental impact.
Acrylamide 2K - Solution (18%) is commonly used in denaturing DNA-PAGE (polyacrylamide gel electrophoresis) to separate DNA fragments based on their size. The acrylamide solution helps to create a gel matrix in which the DNA molecules can migrate through when an electrical current is applied, allowing for the separation and analysis of DNA fragments.
How is Acrylamide 2K - Solution (18%) prepared for use in denaturing DNA-PAGE?
To prepare Acrylamide 2K - Solution (18%) for use in denaturing DNA-PAGE, the solution is typically mixed with a cross-linking agent such as N,N’-methylenebisacrylamide (Bis) and other components to create a gel mixture. This gel mixture is then poured into a mold and allowed to polymerize, forming a solid gel that is ready for the electrophoresis of DNA samples.
What are the advantages of using Acrylamide 2K - Solution (18%) for denaturing DNA-PAGE?
Acrylamide 2K - Solution (18%) offers several advantages for denaturing DNA-PAGE, including high resolution and sharp band separation, making it ideal for the analysis of DNA fragments with similar sizes. The 18% concentration of acrylamide in the solution allows for the separation of small DNA fragments with high accuracy, making it a popular choice for research and diagnostic applications.
Can Acrylamide 2K - Solution (18%) be used for other applications besides denaturing DNA-PAGE?
While Acrylamide 2K - Solution (18%) is primarily used for denaturing DNA-PAGE, it can also be utilized for other applications such as protein electrophoresis and other molecular biology techniques that require the separation of biomolecules based on their size. However, it is important to note that the concentration of acrylamide may need to be adjusted depending on the specific requirements of the experiment.
Is Acrylamide 2K - Solution (18%) safe to use in the laboratory setting?
Acrylamide 2K - Solution (18%) should be handled with care in the laboratory setting, as acrylamide is a known neurotoxin and possible carcinogen. It is important to take necessary precautions when working with acrylamide solutions, such as wearing appropriate personal protective equipment (PPE) and following proper safety protocols. Additionally, disposal of acrylamide waste should be done according to regulations to minimize environmental impact.
