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| Name | Acrylamide 2K - Solution (8 %) |
| Purpose | Denaturing DNA-PAGE |
| Concentration | 8% |
| Appearance | Clear liquid |
| Solubility | Soluble in water |
| Storage | Store at 4°C |
| Shelf Life | 12 months |
| Hazardous | Yes |
| Hazards | May cause skin and eye irritation |
| Usage | For denaturing polyacrylamide gel electrophoresis of DNA samples |
| Preparation | Ready-to-use solution, no additional preparation required |
| Packaging | Available in different volumes (e.g. 100 mL, 500 mL) |
| Compatibility | Compatible with standard DNA electrophoresis buffers |
| Quality Control | Tested for purity and performance |
| Manufacturer | Various suppliers available in the market |
FAQ
What is Acrylamide 2K - Solution (8 %) used for in denaturing DNA-PAGE?
Acrylamide 2K - Solution (8 %) is commonly used in denaturing DNA-PAGE for the separation of DNA fragments based on their size. It helps to create a matrix through which the DNA molecules can migrate, enabling researchers to analyze and visualize the DNA bands.
How does Acrylamide 2K - Solution (8 %) help in denaturing DNA-PAGE?
Acrylamide 2K - Solution (8 %) is a critical component of denaturing DNA-PAGE gels as it forms a polyacrylamide matrix that acts as a sieve to separate DNA fragments based on their size. When an electric current is applied, the DNA molecules move through the gel at different rates depending on their size, facilitating their separation and analysis.
What are the advantages of using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE?
Acrylamide 2K - Solution (8 %) offers several advantages for denaturing DNA-PAGE, including high resolution separation of DNA fragments, compatibility with sensitive detection methods like fluorescent dyes, and the ability to analyze a wide range of DNA fragment sizes. Additionally, the 8% concentration provides a balance between resolution and migration speed, making it ideal for many research applications.
How should Acrylamide 2K - Solution (8 %) be prepared for denaturing DNA-PAGE?
To prepare Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE, follow a standard protocol for making polyacrylamide gels. This typically involves mixing the acrylamide and bis-acrylamide components with a buffer solution, adding a polymerization agent like ammonium persulfate and TEMED, and casting the gel in a suitable mold. After polymerization, the gel is ready for use in denaturing DNA-PAGE experiments.
What considerations should be taken into account when using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE?
When using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE, it is important to handle the solution with caution due to the toxic nature of acrylamide. Proper safety measures should be followed, such as wearing appropriate personal protective equipment and working in a well-ventilated area. Additionally, accurate preparation of the gel and running conditions (voltage, buffer pH, etc.) are essential for obtaining reliable and reproducible results in DNA fragment separation.
Acrylamide 2K - Solution (8 %) is commonly used in denaturing DNA-PAGE for the separation of DNA fragments based on their size. It helps to create a matrix through which the DNA molecules can migrate, enabling researchers to analyze and visualize the DNA bands.
How does Acrylamide 2K - Solution (8 %) help in denaturing DNA-PAGE?
Acrylamide 2K - Solution (8 %) is a critical component of denaturing DNA-PAGE gels as it forms a polyacrylamide matrix that acts as a sieve to separate DNA fragments based on their size. When an electric current is applied, the DNA molecules move through the gel at different rates depending on their size, facilitating their separation and analysis.
What are the advantages of using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE?
Acrylamide 2K - Solution (8 %) offers several advantages for denaturing DNA-PAGE, including high resolution separation of DNA fragments, compatibility with sensitive detection methods like fluorescent dyes, and the ability to analyze a wide range of DNA fragment sizes. Additionally, the 8% concentration provides a balance between resolution and migration speed, making it ideal for many research applications.
How should Acrylamide 2K - Solution (8 %) be prepared for denaturing DNA-PAGE?
To prepare Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE, follow a standard protocol for making polyacrylamide gels. This typically involves mixing the acrylamide and bis-acrylamide components with a buffer solution, adding a polymerization agent like ammonium persulfate and TEMED, and casting the gel in a suitable mold. After polymerization, the gel is ready for use in denaturing DNA-PAGE experiments.
What considerations should be taken into account when using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE?
When using Acrylamide 2K - Solution (8 %) for denaturing DNA-PAGE, it is important to handle the solution with caution due to the toxic nature of acrylamide. Proper safety measures should be followed, such as wearing appropriate personal protective equipment and working in a well-ventilated area. Additionally, accurate preparation of the gel and running conditions (voltage, buffer pH, etc.) are essential for obtaining reliable and reproducible results in DNA fragment separation.
