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| Product Name | Acrylamide - Solution (30%) - Mix 29 : 1 |
| Application | Molecular biology |
| Concentration | 30% |
| Buffer Composition | Tris-HCl, pH 8.8 |
| Storage Condition | Store at room temperature |
| Shelf Life | 12 months |
| Solubility | Soluble in water |
| Appearance | Colorless to slightly yellow liquid |
| Hazards | Toxic by inhalation, ingestion, and skin contact |
| Packaging | Available in various sizes |
| Purity | High purity for molecular biology applications |
FAQ
What is acrylamide solution (30%) used for in molecular biology?
Acrylamide solution (30%) is commonly used in molecular biology for polyacrylamide gel electrophoresis, particularly for protein and nucleic acid separation. It is also used for protein purification and DNA sequencing applications.
How is acrylamide solution (30%) typically prepared for use?
Acrylamide solution (30%) is typically mixed at a ratio of 29:1 with a bis-acrylamide solution to create a polyacrylamide gel. The mixture is then polymerized to form a gel matrix that proteins or nucleic acids can migrate through during electrophoresis.
What are some key advantages of using acrylamide solution (30%) for molecular biology applications?
Acrylamide solution (30%) offers high resolution and sharp band separation for proteins and nucleic acids. It is also relatively easy to prepare and handle, making it a popular choice for research labs.
Are there any safety considerations to keep in mind when working with acrylamide solution (30%)?
Yes, acrylamide is a known neurotoxin and potential carcinogen, so proper safety precautions should be taken when working with acrylamide solution (30%). This includes wearing appropriate personal protective equipment, working in a well-ventilated area, and following safe disposal procedures for unused acrylamide solutions.
Can acrylamide solution (30%) be used for other applications besides gel electrophoresis in molecular biology?
While acrylamide solution (30%) is most commonly used for gel electrophoresis in molecular biology, it can also be used in other fields such as biochemistry and biotechnology for various applications, including protein crystallization and biochemical assays.
Acrylamide solution (30%) is commonly used in molecular biology for polyacrylamide gel electrophoresis, particularly for protein and nucleic acid separation. It is also used for protein purification and DNA sequencing applications.
How is acrylamide solution (30%) typically prepared for use?
Acrylamide solution (30%) is typically mixed at a ratio of 29:1 with a bis-acrylamide solution to create a polyacrylamide gel. The mixture is then polymerized to form a gel matrix that proteins or nucleic acids can migrate through during electrophoresis.
What are some key advantages of using acrylamide solution (30%) for molecular biology applications?
Acrylamide solution (30%) offers high resolution and sharp band separation for proteins and nucleic acids. It is also relatively easy to prepare and handle, making it a popular choice for research labs.
Are there any safety considerations to keep in mind when working with acrylamide solution (30%)?
Yes, acrylamide is a known neurotoxin and potential carcinogen, so proper safety precautions should be taken when working with acrylamide solution (30%). This includes wearing appropriate personal protective equipment, working in a well-ventilated area, and following safe disposal procedures for unused acrylamide solutions.
Can acrylamide solution (30%) be used for other applications besides gel electrophoresis in molecular biology?
While acrylamide solution (30%) is most commonly used for gel electrophoresis in molecular biology, it can also be used in other fields such as biochemistry and biotechnology for various applications, including protein crystallization and biochemical assays.
