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| Product Name | Acrylamide - Solution (40%) - Mix 19:1 |
| Concentration | 40% |
| Mix Ratio | 19 parts acrylamide to 1 part bis-acrylamide |
| Application | Molecular biology |
| Purity | High purity |
| Storage | Store at room temperature |
| Solubility | Soluble in water |
| Appearance | Clear colorless solution |
| Stability | Stable for long-term storage |
| Packaging | Comes in various volumes |
| CAS Number | 79-06-1 |
FAQ
What is acrylamide solution (40%) and how is it used in molecular biology?
Acrylamide solution (40%) is a chemical commonly used in molecular biology for various applications such as protein electrophoresis, DNA sequencing, and polyacrylamide gel casting. It is typically mixed in a 19:1 ratio with other solutions to create a gel matrix that can separate biomolecules based on size and charge.
What are the benefits of using acrylamide solution (40%) in molecular biology experiments?
Acrylamide solution (40%) offers several advantages for molecular biology experiments, including high resolution separation of biomolecules, reproducibility, and compatibility with various detection methods such as UV, Coomassie staining, and fluorescent dyes. Additionally, acrylamide gels can be easily adapted for different applications by adjusting the acrylamide concentration and buffer composition.
How do I prepare a acrylamide gel using acrylamide solution (40%)?
To prepare an acrylamide gel using acrylamide solution (40%), first mix the acrylamide solution with other components such as bis-acrylamide, water, and a buffer to achieve the desired acrylamide concentration. Then, add a crosslinking agent such as ammonium persulfate and TEMED to initiate polymerization. Pour the gel mixture into a casting tray and insert a comb to create wells for sample loading. Allow the gel to polymerize and then remove the comb before running samples.
What safety precautions should be followed when handling acrylamide solution (40%) in the laboratory?
When handling acrylamide solution (40%) in the laboratory, it is important to wear appropriate personal protective equipment such as gloves, lab coat, and safety goggles to prevent skin contact and inhalation of fumes. Acrylamide is a neurotoxin and a potential carcinogen, so it should be used in a well-ventilated area to minimize exposure. Spills should be cleaned up immediately using absorbent materials and proper disposal methods should be followed.
Can acrylamide solution (40%) be stored for long periods of time and how should it be stored?
Acrylamide solution (40%) should be stored in a cool, dark place away from direct sunlight and heat sources to prevent degradation. It is recommended to store acrylamide solution at 4°C to prolong its shelf life. Before using stored acrylamide solution, it is important to check for any signs of deterioration such as discoloration or precipitation. If any such signs are present, the solution should be discarded and a fresh batch should be prepared.
Acrylamide solution (40%) is a chemical commonly used in molecular biology for various applications such as protein electrophoresis, DNA sequencing, and polyacrylamide gel casting. It is typically mixed in a 19:1 ratio with other solutions to create a gel matrix that can separate biomolecules based on size and charge.
What are the benefits of using acrylamide solution (40%) in molecular biology experiments?
Acrylamide solution (40%) offers several advantages for molecular biology experiments, including high resolution separation of biomolecules, reproducibility, and compatibility with various detection methods such as UV, Coomassie staining, and fluorescent dyes. Additionally, acrylamide gels can be easily adapted for different applications by adjusting the acrylamide concentration and buffer composition.
How do I prepare a acrylamide gel using acrylamide solution (40%)?
To prepare an acrylamide gel using acrylamide solution (40%), first mix the acrylamide solution with other components such as bis-acrylamide, water, and a buffer to achieve the desired acrylamide concentration. Then, add a crosslinking agent such as ammonium persulfate and TEMED to initiate polymerization. Pour the gel mixture into a casting tray and insert a comb to create wells for sample loading. Allow the gel to polymerize and then remove the comb before running samples.
What safety precautions should be followed when handling acrylamide solution (40%) in the laboratory?
When handling acrylamide solution (40%) in the laboratory, it is important to wear appropriate personal protective equipment such as gloves, lab coat, and safety goggles to prevent skin contact and inhalation of fumes. Acrylamide is a neurotoxin and a potential carcinogen, so it should be used in a well-ventilated area to minimize exposure. Spills should be cleaned up immediately using absorbent materials and proper disposal methods should be followed.
Can acrylamide solution (40%) be stored for long periods of time and how should it be stored?
Acrylamide solution (40%) should be stored in a cool, dark place away from direct sunlight and heat sources to prevent degradation. It is recommended to store acrylamide solution at 4°C to prolong its shelf life. Before using stored acrylamide solution, it is important to check for any signs of deterioration such as discoloration or precipitation. If any such signs are present, the solution should be discarded and a fresh batch should be prepared.
