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| Product Name | Acrylamide Solution (40%) - Mix 29:1 |
| Product Type | Molecular Biology Reagent |
| Concentration | 40% |
| Solvent | Water |
| Storage Condition | Store at 4°C |
| Purity | High purity |
| Application | Molecular biology studies |
| Stability | Stable for long-term storage |
| Packaging Size | 500 mL |
| Shipping Condition | Shipped at room temperature |
| Handling Precautions | Avoid contact with skin and eyes |
| Shelf Life | 6 months |
| Supplier | Major molecular biology reagent supplier |
| Catalog Number | ABC123 |
| Recommended Working Solution Concentration | 2% |
| Storage Stability | Stable at 4°C for multiple freeze-thaw cycles |
| Compatibility | Compatible with various molecular biology techniques |
| Recommended Storage Temperature | 4°C |
FAQ
What is acrylamide solution (40%) and its application in molecular biology?
Acrylamide solution (40%) is a powerful denaturant used in molecular biology for separating nucleic acids and proteins in gel electrophoresis. It is commonly mixed with urea in a ratio of 29:1 to form denaturing solutions for a variety of applications in research and diagnostics.
How is acrylamide solution (40%) used in gel electrophoresis?
Acrylamide solution (40%) is mixed with other components such as urea, Tris buffer, and ammonium persulfate to create denaturing gels for gel electrophoresis. These gels are used to separate nucleic acids or proteins based on size and charge under an electrical field.
What are the advantages of using acrylamide solution (40%) in gel electrophoresis?
Acrylamide solution (40%) provides high resolution and sharp banding patterns in gel electrophoresis compared to agarose gels. It allows for separation of smaller nucleic acids or proteins with high precision and resolution.
Are there any safety precautions to be followed while handling acrylamide solution (40%)?
Yes, acrylamide is a known neurotoxin and carcinogen, so it should be handled with caution. Always wear appropriate personal protective equipment, such as gloves and lab coat, and work in a well-ventilated area. Minimize skin contact and inhalation exposure to avoid any health risks.
Can acrylamide solution (40%) be stored for long periods? What are the storage conditions?
Acrylamide solution (40%) should be stored at 4°C to maintain its stability. It is recommended to aliquot the solution into smaller volumes to avoid repeated freeze-thaw cycles which may affect its performance. Properly stored acrylamide solution can be used for several months in the laboratory.
Acrylamide solution (40%) is a powerful denaturant used in molecular biology for separating nucleic acids and proteins in gel electrophoresis. It is commonly mixed with urea in a ratio of 29:1 to form denaturing solutions for a variety of applications in research and diagnostics.
How is acrylamide solution (40%) used in gel electrophoresis?
Acrylamide solution (40%) is mixed with other components such as urea, Tris buffer, and ammonium persulfate to create denaturing gels for gel electrophoresis. These gels are used to separate nucleic acids or proteins based on size and charge under an electrical field.
What are the advantages of using acrylamide solution (40%) in gel electrophoresis?
Acrylamide solution (40%) provides high resolution and sharp banding patterns in gel electrophoresis compared to agarose gels. It allows for separation of smaller nucleic acids or proteins with high precision and resolution.
Are there any safety precautions to be followed while handling acrylamide solution (40%)?
Yes, acrylamide is a known neurotoxin and carcinogen, so it should be handled with caution. Always wear appropriate personal protective equipment, such as gloves and lab coat, and work in a well-ventilated area. Minimize skin contact and inhalation exposure to avoid any health risks.
Can acrylamide solution (40%) be stored for long periods? What are the storage conditions?
Acrylamide solution (40%) should be stored at 4°C to maintain its stability. It is recommended to aliquot the solution into smaller volumes to avoid repeated freeze-thaw cycles which may affect its performance. Properly stored acrylamide solution can be used for several months in the laboratory.
