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| Name | Acrylamide - Solution (40%) - Mix 37.5 : 1 |
| Purity | 40% |
| Ratio | 37.5 : 1 |
| Form | Solution |
| Application | Molecular biology |
| Storage Condition | Store at 2-8°C |
| Solubility | Soluble in water |
| Appearance | Clear colorless to slightly yellow solution |
| Cas Number | 79-06-1 |
| Molecular Weight | 71.08 g/mol |
| Boiling Point | 125°C |
| Melting Point | 84°C |
FAQ
What is Acrylamide - Solution (40%) - Mix 37.5:1 used for in molecular
biology?
Acrylamide - Solution (40%) - Mix 37.5:1 is commonly used in molecular biology for protein electrophoresis. It is used in the production of polyacrylamide gels for techniques such as SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and native PAGE (Polyacrylamide Gel Electrophoresis).
How is Acrylamide - Solution (40%) - Mix 37.5:1 prepared for use in protein electrophoresis?
To prepare Acrylamide - Solution (40%) - Mix 37.5:1 for protein electrophoresis, the solution is diluted with water and other components such as bis-acrylamide, ammonium persulfate, and TEMED are added to initiate the polymerization process. The mixture is then poured into a gel casting apparatus and allowed to polymerize to form a gel suitable for protein separation.
What are the advantages of using Acrylamide - Solution (40%) - Mix 37.5:1 for protein electrophoresis?
Acrylamide - Solution (40%) - Mix 37.5:1 offers several advantages for protein electrophoresis. It provides a high degree of resolution, allowing for the separation of proteins based on size and charge. The gel is stable and can withstand the rigors of electrophoresis without breaking down. Additionally, the consistency of the gel allows for reproducible results, making it ideal for research applications.
Are there any safety considerations when working with Acrylamide - Solution (40%) - Mix 37.5:1?
When working with Acrylamide - Solution (40%) - Mix 37.5:1, it is important to take appropriate safety precautions. Acrylamide is a neurotoxin and a potential carcinogen, so it is essential to wear appropriate personal protective equipment, such as gloves and a lab coat, when handling the solution. Additionally, solutions containing acrylamide should be prepared in a fume hood to minimize exposure to vapors.
Can Acrylamide - Solution (40%) - Mix 37.5:1 be used for other applications besides protein electrophoresis?
While Acrylamide - Solution (40%) - Mix 37.5:1 is primarily used for protein electrophoresis, it can also be used in other applications such as DNA sequencing and molecular biology research. The versatility of acrylamide gels makes them suitable for a variety of techniques that require the separation of biomolecules based on size or charge.
Acrylamide - Solution (40%) - Mix 37.5:1 is commonly used in molecular biology for protein electrophoresis. It is used in the production of polyacrylamide gels for techniques such as SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) and native PAGE (Polyacrylamide Gel Electrophoresis).
How is Acrylamide - Solution (40%) - Mix 37.5:1 prepared for use in protein electrophoresis?
To prepare Acrylamide - Solution (40%) - Mix 37.5:1 for protein electrophoresis, the solution is diluted with water and other components such as bis-acrylamide, ammonium persulfate, and TEMED are added to initiate the polymerization process. The mixture is then poured into a gel casting apparatus and allowed to polymerize to form a gel suitable for protein separation.
What are the advantages of using Acrylamide - Solution (40%) - Mix 37.5:1 for protein electrophoresis?
Acrylamide - Solution (40%) - Mix 37.5:1 offers several advantages for protein electrophoresis. It provides a high degree of resolution, allowing for the separation of proteins based on size and charge. The gel is stable and can withstand the rigors of electrophoresis without breaking down. Additionally, the consistency of the gel allows for reproducible results, making it ideal for research applications.
Are there any safety considerations when working with Acrylamide - Solution (40%) - Mix 37.5:1?
When working with Acrylamide - Solution (40%) - Mix 37.5:1, it is important to take appropriate safety precautions. Acrylamide is a neurotoxin and a potential carcinogen, so it is essential to wear appropriate personal protective equipment, such as gloves and a lab coat, when handling the solution. Additionally, solutions containing acrylamide should be prepared in a fume hood to minimize exposure to vapors.
Can Acrylamide - Solution (40%) - Mix 37.5:1 be used for other applications besides protein electrophoresis?
While Acrylamide - Solution (40%) - Mix 37.5:1 is primarily used for protein electrophoresis, it can also be used in other applications such as DNA sequencing and molecular biology research. The versatility of acrylamide gels makes them suitable for a variety of techniques that require the separation of biomolecules based on size or charge.
