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| Product Name | Bisacrylamide |
| Product Type | Chemical reagent |
| Use | Used in molecular biology for nucleic acid and protein electrophoresis |
| Appearance | White powder |
| Solubility | Soluble in water |
| Purity | ≥99% |
| Storage | Store at room temperature in a dry place |
| Melting Point | 160-163°C |
| Molecular Formula | C7H10N2O2 |
| Molecular Weight | 154.17 g/mol |
FAQ
What is bisacrylamide?
Bisacrylamide is a chemical compound used in molecular biology for the formation of polyacrylamide gels. It is a crosslinking agent that helps to stabilize the gel matrix, allowing for the separation of DNA, RNA, and proteins based on size.
How is bisacrylamide used in molecular biology?
Bisacrylamide is typically used in conjunction with acrylamide to create polyacrylamide gels for techniques such as gel electrophoresis. The concentration of bisacrylamide in the gel determines the pore size and therefore the resolution of the separation.
What are the benefits of using bisacrylamide in molecular biology applications?
Bisacrylamide offers several advantages in molecular biology applications. It provides a high degree of flexibility in adjusting the gel properties to suit specific experimental needs. Additionally, bisacrylamide gels are compatible with a wide range of detection methods and can be used for both analytical and preparative purposes.
Are there any safety considerations when working with bisacrylamide?
Yes, bisacrylamide is a hazardous chemical and should be handled with care. It is important to wear appropriate personal protective equipment, such as gloves and lab coats, when working with bisacrylamide. In addition, proper disposal methods should be followed to minimize environmental impact.
What are some common applications of bisacrylamide in molecular biology research?
Bisacrylamide is commonly used in molecular biology research for a variety of applications, including DNA sequencing, protein electrophoresis, and Western blotting. It is a versatile tool that plays a key role in the analysis and characterization of biological molecules.
Bisacrylamide is a chemical compound used in molecular biology for the formation of polyacrylamide gels. It is a crosslinking agent that helps to stabilize the gel matrix, allowing for the separation of DNA, RNA, and proteins based on size.
How is bisacrylamide used in molecular biology?
Bisacrylamide is typically used in conjunction with acrylamide to create polyacrylamide gels for techniques such as gel electrophoresis. The concentration of bisacrylamide in the gel determines the pore size and therefore the resolution of the separation.
What are the benefits of using bisacrylamide in molecular biology applications?
Bisacrylamide offers several advantages in molecular biology applications. It provides a high degree of flexibility in adjusting the gel properties to suit specific experimental needs. Additionally, bisacrylamide gels are compatible with a wide range of detection methods and can be used for both analytical and preparative purposes.
Are there any safety considerations when working with bisacrylamide?
Yes, bisacrylamide is a hazardous chemical and should be handled with care. It is important to wear appropriate personal protective equipment, such as gloves and lab coats, when working with bisacrylamide. In addition, proper disposal methods should be followed to minimize environmental impact.
What are some common applications of bisacrylamide in molecular biology research?
Bisacrylamide is commonly used in molecular biology research for a variety of applications, including DNA sequencing, protein electrophoresis, and Western blotting. It is a versatile tool that plays a key role in the analysis and characterization of biological molecules.
