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| Product Name | Chymostatin |
| Type | Protease Inhibitor |
| Target | Chymotrypsin |
| Species Specificity | Broad Spectrum (Mammalian enzymes) |
| CAS Number | 9076-44-2 |
| Molecular Formula | C77H119N17O21 |
| Molecular Weight | 1536.88 g/mol |
| Solubility | Soluble in water and DMSO |
| Storage Conditions | Store at -20°C |
| Appearance | White to off-white solid |
| Purity | ≥98% by HPLC |
| Usage | Biochemical research, enzyme inhibition studies |
| References | Bhaskara Reddy et al. (2015) J Mol Catal B Enzym 121: 124-132 |
| Supplier | Sigma-Aldrich |
FAQ
What is Chymostatin and how does it work?
Chymostatin is a powerful protease inhibitor that is commonly used in biological and biochemical research. It works by binding to the active site of enzymes called chymotrypsins, which are responsible for breaking down proteins in the body. By inhibiting these enzymes, Chymostatin can prevent the degradation of proteins and help researchers study their structure and function.
What are the primary applications of Chymostatin?
Chymostatin is commonly used in research to study the role of chymotrypsins in various biological processes. It can also be used to investigate the structure and function of proteins, as well as to study the mechanisms of enzyme inhibition. Additionally, Chymostatin has been used in cell culture studies to protect cells from protease activity and improve protein stability.
How is Chymostatin typically used in research experiments?
In research experiments, Chymostatin is usually dissolved in a suitable solvent, such as dimethyl sulfoxide (DMSO), and added to the experimental samples at a specific concentration. Researchers can then incubate their samples with Chymostatin for a desired period of time to inhibit chymotrypsin activity. The effectiveness of Chymostatin can be assessed by measuring the remaining enzyme activity or protein degradation in the samples.
What are the advantages of using Chymostatin in research?
One of the main advantages of using Chymostatin in research is its specificity for chymotrypsin enzymes, which allows researchers to selectively inhibit their activity without affecting other types of proteases. This can help researchers study the role of chymotrypsins in specific biological pathways or processes. Additionally, Chymostatin is stable and easy to use, making it a convenient tool for studying protein degradation and enzyme inhibition.
Are there any limitations or considerations when using Chymostatin in research?
While Chymostatin is a valuable tool in research, there are some limitations and considerations to keep in mind. For example, Chymostatin may not be effective at inhibiting all types of chymotrypsins, so researchers should carefully select their experimental conditions and concentrations. Additionally, Chymostatin can be toxic to cells at high concentrations, so researchers should optimize their experimental conditions to minimize any potential effects on cell viability.
Chymostatin is a powerful protease inhibitor that is commonly used in biological and biochemical research. It works by binding to the active site of enzymes called chymotrypsins, which are responsible for breaking down proteins in the body. By inhibiting these enzymes, Chymostatin can prevent the degradation of proteins and help researchers study their structure and function.
What are the primary applications of Chymostatin?
Chymostatin is commonly used in research to study the role of chymotrypsins in various biological processes. It can also be used to investigate the structure and function of proteins, as well as to study the mechanisms of enzyme inhibition. Additionally, Chymostatin has been used in cell culture studies to protect cells from protease activity and improve protein stability.
How is Chymostatin typically used in research experiments?
In research experiments, Chymostatin is usually dissolved in a suitable solvent, such as dimethyl sulfoxide (DMSO), and added to the experimental samples at a specific concentration. Researchers can then incubate their samples with Chymostatin for a desired period of time to inhibit chymotrypsin activity. The effectiveness of Chymostatin can be assessed by measuring the remaining enzyme activity or protein degradation in the samples.
What are the advantages of using Chymostatin in research?
One of the main advantages of using Chymostatin in research is its specificity for chymotrypsin enzymes, which allows researchers to selectively inhibit their activity without affecting other types of proteases. This can help researchers study the role of chymotrypsins in specific biological pathways or processes. Additionally, Chymostatin is stable and easy to use, making it a convenient tool for studying protein degradation and enzyme inhibition.
Are there any limitations or considerations when using Chymostatin in research?
While Chymostatin is a valuable tool in research, there are some limitations and considerations to keep in mind. For example, Chymostatin may not be effective at inhibiting all types of chymotrypsins, so researchers should carefully select their experimental conditions and concentrations. Additionally, Chymostatin can be toxic to cells at high concentrations, so researchers should optimize their experimental conditions to minimize any potential effects on cell viability.
