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| Name | D-Luciferin Sodium Salt |
| CAS Number | 115144-35-9 |
| Molecular Formula | C11H7N2O3SNa |
| Molecular Weight | 292.24 g/mol |
| Appearance | Off-white to pale yellow solid |
| Solubility | Soluble in water |
| Purity | ≥98% |
| Storage | Store at -20°C |
| Stability | Stable for at least 2 years when stored properly |
| Usage | Substrate for firefly luciferase |
| Applications | Bioluminescence imaging, ATP detection, reporter gene assays |
FAQ
What is D-Luciferin Sodium Salt and how is it used in research?
D-Luciferin Sodium Salt is a substrate for the enzyme luciferase, which is commonly used as a reporter in bioluminescence imaging studies. When luciferin is oxidized by luciferase, it emits light that can be detected and measured using specialized equipment. This makes D-Luciferin Sodium Salt particularly useful for studying gene expression, protein-protein interactions, and in vivo imaging of biological processes in living organisms.
Is D-Luciferin Sodium Salt compatible with different imaging modalities?
Yes, D-Luciferin Sodium Salt is compatible with a variety of imaging modalities, including bioluminescence imaging, fluorescence imaging, and positron emission tomography (PET). Researchers can choose the imaging technique that best suits their experimental needs and use D-Luciferin Sodium Salt as a versatile tool for visualizing and quantifying biological processes in real time.
What are the advantages of using D-Luciferin Sodium Salt in research?
One of the main advantages of D-Luciferin Sodium Salt is its high sensitivity, allowing researchers to detect low levels of luciferase activity with high signal-to-noise ratios. Additionally, D-Luciferin Sodium Salt is non-toxic and does not require cell lysis or fixation, making it ideal for longitudinal studies and monitoring dynamic biological processes over time. Its compatibility with various imaging modalities further enhances its versatility and applicability in a wide range of research fields.
How can D-Luciferin Sodium Salt benefit my research?
Whether you are studying gene expression, protein-protein interactions, or the efficacy of therapeutic interventions, D-Luciferin Sodium Salt can provide valuable insights into the underlying mechanisms of biological processes. By visualizing and quantifying luciferase activity in real time, researchers can track changes over time, assess treatment outcomes, and gain a deeper understanding of the molecular events occurring within cells and tissues. This can lead to new discoveries, innovative therapies, and improved research outcomes in a variety of scientific disciplines.
Are there any specific considerations to keep in mind when using D-Luciferin Sodium Salt in research?
While D-Luciferin Sodium Salt is a powerful tool for studying biological processes, researchers should be aware of the potential limitations and challenges associated with its use. For example, factors such as pH, temperature, and substrate concentration can affect the sensitivity and specificity of luciferase assays. It is important to optimize experimental conditions, control for potential variables, and validate results using appropriate controls and standards. Additionally, researchers should follow best practices for handling and storing D-Luciferin Sodium Salt to ensure its stability and efficacy throughout the course of their experiments.
D-Luciferin Sodium Salt is a substrate for the enzyme luciferase, which is commonly used as a reporter in bioluminescence imaging studies. When luciferin is oxidized by luciferase, it emits light that can be detected and measured using specialized equipment. This makes D-Luciferin Sodium Salt particularly useful for studying gene expression, protein-protein interactions, and in vivo imaging of biological processes in living organisms.
Is D-Luciferin Sodium Salt compatible with different imaging modalities?
Yes, D-Luciferin Sodium Salt is compatible with a variety of imaging modalities, including bioluminescence imaging, fluorescence imaging, and positron emission tomography (PET). Researchers can choose the imaging technique that best suits their experimental needs and use D-Luciferin Sodium Salt as a versatile tool for visualizing and quantifying biological processes in real time.
What are the advantages of using D-Luciferin Sodium Salt in research?
One of the main advantages of D-Luciferin Sodium Salt is its high sensitivity, allowing researchers to detect low levels of luciferase activity with high signal-to-noise ratios. Additionally, D-Luciferin Sodium Salt is non-toxic and does not require cell lysis or fixation, making it ideal for longitudinal studies and monitoring dynamic biological processes over time. Its compatibility with various imaging modalities further enhances its versatility and applicability in a wide range of research fields.
How can D-Luciferin Sodium Salt benefit my research?
Whether you are studying gene expression, protein-protein interactions, or the efficacy of therapeutic interventions, D-Luciferin Sodium Salt can provide valuable insights into the underlying mechanisms of biological processes. By visualizing and quantifying luciferase activity in real time, researchers can track changes over time, assess treatment outcomes, and gain a deeper understanding of the molecular events occurring within cells and tissues. This can lead to new discoveries, innovative therapies, and improved research outcomes in a variety of scientific disciplines.
Are there any specific considerations to keep in mind when using D-Luciferin Sodium Salt in research?
While D-Luciferin Sodium Salt is a powerful tool for studying biological processes, researchers should be aware of the potential limitations and challenges associated with its use. For example, factors such as pH, temperature, and substrate concentration can affect the sensitivity and specificity of luciferase assays. It is important to optimize experimental conditions, control for potential variables, and validate results using appropriate controls and standards. Additionally, researchers should follow best practices for handling and storing D-Luciferin Sodium Salt to ensure its stability and efficacy throughout the course of their experiments.
