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| Name | IPTG for Molecular Biology, Dioxane Free |
| Purity | ≥99.0% |
| Appearance | White to off-white crystalline powder |
| Solubility | Clear, colorless solution at 100 mg/ml in water |
| Stability | Stable at -20°C |
| Storage | Store at -20°C |
| CAS Number | 367-93-1 |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.30 g/mol |
| Melting Point | 93-95°C |
| Applications | Inducer of β-galactosidase activity in bacteria |
FAQ
What is IPTG and how is it used in molecular biology?
IPTG, or isopropyl ß-D-1-thiogalactopyranoside, is a molecular biology reagent commonly used to induce protein expression in bacterial cells. It is an analog of lactose, and it is a commonly used chemical for this purpose because it is non-metabolizable by most bacteria. IPTG is used in conjunction with plasmid vectors that contain the lac operon to enable the controlled expression of a gene of interest.
Why is it important for IPTG to be dioxane-free?
Dioxane is a toxic and hazardous chemical that can have detrimental effects on both human health and the environment. Any residual dioxane in molecular biology reagents, including IPTG, can potentially contaminate experiments and have unintended consequences on results. Therefore, it is crucial for IPTG used in molecular biology research to be dioxane-free to ensure the safety and reliability of experimental outcomes.
How do researchers typically use IPTG in molecular biology experiments?
Researchers use IPTG to induce protein expression in bacterial cells that have been transformed with a plasmid containing the gene of interest under the control of the lac promoter. IPTG acts as an inducer, mimicking the natural substrate of the lac repressor protein, and thereby induces the expression of the target protein. By adding IPTG to the growth medium, researchers can control when and how much of the target protein is produced by the bacterial cells.
What are some advantages of using IPTG for protein expression as opposed to other inducers?
IPTG is a widely used inducer for protein expression in bacterial systems due to its non-metabolizable nature, which allows for tight regulation of the induction process. IPTG is also relatively inexpensive and easy to use, making it a popular choice for researchers working with bacterial expression systems. Additionally, IPTG induction is typically rapid and highly efficient, resulting in high levels of protein expression.
Are there any precautions that researchers should take when working with IPTG in the lab?
When working with IPTG in the lab, researchers should take standard precautions for handling chemicals, including wearing appropriate personal protective equipment such as gloves and eye protection. It is also important to properly label IPTG containers and store them in a cool, dry place away from direct sunlight. Researchers should be mindful of the concentration of IPTG used in experiments, as higher concentrations can lead to non-specific effects or toxicity in bacterial cells.
IPTG, or isopropyl ß-D-1-thiogalactopyranoside, is a molecular biology reagent commonly used to induce protein expression in bacterial cells. It is an analog of lactose, and it is a commonly used chemical for this purpose because it is non-metabolizable by most bacteria. IPTG is used in conjunction with plasmid vectors that contain the lac operon to enable the controlled expression of a gene of interest.
Why is it important for IPTG to be dioxane-free?
Dioxane is a toxic and hazardous chemical that can have detrimental effects on both human health and the environment. Any residual dioxane in molecular biology reagents, including IPTG, can potentially contaminate experiments and have unintended consequences on results. Therefore, it is crucial for IPTG used in molecular biology research to be dioxane-free to ensure the safety and reliability of experimental outcomes.
How do researchers typically use IPTG in molecular biology experiments?
Researchers use IPTG to induce protein expression in bacterial cells that have been transformed with a plasmid containing the gene of interest under the control of the lac promoter. IPTG acts as an inducer, mimicking the natural substrate of the lac repressor protein, and thereby induces the expression of the target protein. By adding IPTG to the growth medium, researchers can control when and how much of the target protein is produced by the bacterial cells.
What are some advantages of using IPTG for protein expression as opposed to other inducers?
IPTG is a widely used inducer for protein expression in bacterial systems due to its non-metabolizable nature, which allows for tight regulation of the induction process. IPTG is also relatively inexpensive and easy to use, making it a popular choice for researchers working with bacterial expression systems. Additionally, IPTG induction is typically rapid and highly efficient, resulting in high levels of protein expression.
Are there any precautions that researchers should take when working with IPTG in the lab?
When working with IPTG in the lab, researchers should take standard precautions for handling chemicals, including wearing appropriate personal protective equipment such as gloves and eye protection. It is also important to properly label IPTG containers and store them in a cool, dry place away from direct sunlight. Researchers should be mindful of the concentration of IPTG used in experiments, as higher concentrations can lead to non-specific effects or toxicity in bacterial cells.
