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| Name | Leupeptin Hemisulfate |
| Product Type | Protease inhibitor |
| Form | Powder |
| Purity | ≥98% |
| Solubility | Soluble in water and DMSO |
| Storage Conditions | Store at -20°C |
| Molecular Weight | 605.73 g/mol |
| CAS Number | 103476-89-7 |
| Appearance | White to off-white crystalline powder |
| Usage | Used for the inhibition of proteases in biological samples |
| Biological Activity | Inhibits serine and cysteine proteases |
| Source | Synthetic |
FAQ
What is Leupeptin Hemisulfate and how does it work?
Leupeptin Hemisulfate is a protease inhibitor commonly used in laboratory research to prevent protein degradation. It works by inhibiting proteases, enzymes that break down proteins by cleaving peptide bonds. This helps researchers maintain the integrity of their protein samples and study their functions without interference from proteolytic activity.
What are the main applications of Leupeptin Hemisulfate in research?
Leupeptin Hemisulfate is commonly used in various research applications involving protein isolation, purification, and analysis. It is particularly useful in studies that require the preservation of protein integrity, such as enzyme assays, western blotting, and protein crystallization. Additionally, Leupeptin Hemisulfate is often included in cell lysis buffers to prevent protein degradation during sample preparation for downstream experiments.
How should Leupeptin Hemisulfate be stored and handled to maintain its stability and efficacy?
Leupeptin Hemisulfate should be stored at -20°C in a dark, dry place to prevent degradation. It is also important to protect the compound from light exposure, as it is sensitive to photodegradation. When handling Leupeptin Hemisulfate, researchers should wear appropriate personal protective equipment, such as gloves and lab coats, to avoid direct skin contact. Additionally, solutions of Leupeptin Hemisulfate should be prepared fresh before use to ensure maximum efficacy.
Are there any specific precautions or considerations to keep in mind when using Leupeptin Hemisulfate in experiments?
Researchers should be aware that Leupeptin Hemisulfate is not stable in acidic conditions, as it can undergo hydrolysis and lose its inhibitory activity. Therefore, it is important to maintain a neutral pH when working with solutions containing Leupeptin Hemisulfate. Furthermore, since Leupeptin Hemisulfate is a potent protease inhibitor, it is essential to avoid contamination with other proteases during experimental procedures to prevent unwanted proteolysis of proteins of interest.
What makes Leupeptin Hemisulfate a valuable tool for protein research compared to other protease inhibitors?
Leupeptin Hemisulfate is a reversible inhibitor of a broad spectrum of proteases, including serine, cysteine, and metalloproteases. This feature makes it a versatile tool for researchers working with a variety of protein samples. In addition, Leupeptin Hemisulfate is known for its stability and compatibility with other laboratory reagents, making it easy to incorporate into existing experimental protocols without significant adjustments. Overall, Leupeptin Hemisulfate is a reliable and effective protease inhibitor that is widely used in protein research for its convenience and superior performance.
Leupeptin Hemisulfate is a protease inhibitor commonly used in laboratory research to prevent protein degradation. It works by inhibiting proteases, enzymes that break down proteins by cleaving peptide bonds. This helps researchers maintain the integrity of their protein samples and study their functions without interference from proteolytic activity.
What are the main applications of Leupeptin Hemisulfate in research?
Leupeptin Hemisulfate is commonly used in various research applications involving protein isolation, purification, and analysis. It is particularly useful in studies that require the preservation of protein integrity, such as enzyme assays, western blotting, and protein crystallization. Additionally, Leupeptin Hemisulfate is often included in cell lysis buffers to prevent protein degradation during sample preparation for downstream experiments.
How should Leupeptin Hemisulfate be stored and handled to maintain its stability and efficacy?
Leupeptin Hemisulfate should be stored at -20°C in a dark, dry place to prevent degradation. It is also important to protect the compound from light exposure, as it is sensitive to photodegradation. When handling Leupeptin Hemisulfate, researchers should wear appropriate personal protective equipment, such as gloves and lab coats, to avoid direct skin contact. Additionally, solutions of Leupeptin Hemisulfate should be prepared fresh before use to ensure maximum efficacy.
Are there any specific precautions or considerations to keep in mind when using Leupeptin Hemisulfate in experiments?
Researchers should be aware that Leupeptin Hemisulfate is not stable in acidic conditions, as it can undergo hydrolysis and lose its inhibitory activity. Therefore, it is important to maintain a neutral pH when working with solutions containing Leupeptin Hemisulfate. Furthermore, since Leupeptin Hemisulfate is a potent protease inhibitor, it is essential to avoid contamination with other proteases during experimental procedures to prevent unwanted proteolysis of proteins of interest.
What makes Leupeptin Hemisulfate a valuable tool for protein research compared to other protease inhibitors?
Leupeptin Hemisulfate is a reversible inhibitor of a broad spectrum of proteases, including serine, cysteine, and metalloproteases. This feature makes it a versatile tool for researchers working with a variety of protein samples. In addition, Leupeptin Hemisulfate is known for its stability and compatibility with other laboratory reagents, making it easy to incorporate into existing experimental protocols without significant adjustments. Overall, Leupeptin Hemisulfate is a reliable and effective protease inhibitor that is widely used in protein research for its convenience and superior performance.
