PMSF BioChemica

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Name PMSF BioChemica
Supplier MilliporeSigma
Product Type Protease Inhibitor
Purity ≥98%
Molecular Formula C7H7FO2S
Molecular Weight 174.20 g/mol
Solubility Soluble in ethanol, isopropanol, and acetone
Storage Condition Store at -20°C
Appearance White crystalline powder
CAS Number 329-98-6
Usage Used to inhibit serine proteases in protein purification and cell culture applications
FAQ
What is PMSF BioChemica and how does it work?

PMSF BioChemica is a potent inhibitor of serine proteases and is commonly used in biochemistry laboratories to inhibit these enzymes. Its mechanism of action involves reacting with the hydroxyl group present in the active site of the enzyme, resulting in the formation of a stable, covalent bond. This effectively inactivates the enzyme and prevents it from carrying out its normal function.

What are the main applications of PMSF BioChemica?

PMSF BioChemica is widely used in various biochemical and molecular biology applications due to its ability to selectively inhibit serine proteases. Some common applications include the inhibition of proteases in protein purification procedures, the prevention of protein degradation during sample preparation, and the protection of proteins from proteolytic degradation during downstream processes such as Western blotting and enzyme assays.

How should PMSF BioChemica be used in a laboratory setting?

PMSF BioChemica should be handled with care in the laboratory, as it is a potent inhibitor of serine proteases. It is typically dissolved in a suitable solvent, such as ethanol or isopropanol, to prepare a stock solution of the desired concentration. The stock solution can then be added to the sample or reaction mixture to achieve the desired level of enzyme inhibition. It is important to note that PMSF BioChemica is sensitive to pH and temperature, so it is recommended to store the stock solution at -20°C and prepare fresh solutions before each use.

Are there any precautions that should be taken when working with PMSF BioChemica?

Yes, there are several precautions that should be taken when working with PMSF BioChemica in the laboratory. It is important to wear appropriate personal protective equipment, such as gloves and safety goggles, when handling the compound. Additionally, PMSF BioChemica should be stored in a cool, dry place away from sources of heat and light to prevent degradation. Care should also be taken to avoid inhaling the compound or allowing it to come into contact with skin or mucous membranes, as it can be irritating and toxic if absorbed.

What are the advantages of using PMSF BioChemica in biochemical research?

PMSF BioChemica offers several advantages for researchers working in the field of biochemistry. Its high potency and selectivity make it an effective inhibitor of serine proteases, allowing for specific and targeted enzyme inhibition. Additionally, PMSF BioChemica is relatively easy to use and can be quickly incorporated into standard laboratory protocols. Its stability and compatibility with a wide range of solvents also make it a versatile tool for studying protease activity and protein degradation processes.
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