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| Product Name | Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica |
| Organism | Chemical compound |
| Appearance | Yellowish brown powder |
| Solubility | Soluble in water |
| Storage | Store at room temperature |
| Shelf Life | At least 2 years |
| Use | Cell viability and proliferation assay |
| Purity | ≥98% |
| Melting Point | >300°C |
| Absorption Max | 565 nm |
| Stability | Stable under normal conditions |
| Special Precautions | Avoid contact with skin and eyes |
| Applications | Used in biochemical research and drug discovery |
FAQ
What is Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica?
Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica is a commonly used compound in biochemistry and cell biology research. It is a yellow tetrazolium salt that is used to measure cell viability and proliferation in cell culture experiments. MTT is converted into a purple formazan product by living cells, allowing researchers to quantify cell growth and metabolic activity.
How is Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica used in research?
MTT is typically used in cell viability assays to assess the effects of various treatments or conditions on cell health. After treating cells with a compound or subjecting them to specific conditions, researchers add MTT to the cell culture medium. Live cells reduce MTT to formazan, which can be quantified using spectrophotometry. The amount of formazan produced is directly proportional to the number of viable cells in the culture.
What are the advantages of using Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica?
One of the main advantages of using MTT in cell viability assays is its simplicity and reliability. The assay can be performed relatively quickly and does not require specialized equipment. Additionally, MTT is non-toxic to cells, allowing for repeated measurements over time. The results obtained with MTT are also highly reproducible, making it a preferred choice for many researchers in the field.
Can Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica be used in combination with other assays?
Yes, MTT can be used in combination with other assays to obtain more comprehensive information about cell viability and proliferation. For example, researchers often perform MTT assays alongside other assays such as lactate dehydrogenase (LDH) release assays to assess cytotoxicity. Combining different assays can provide a more thorough understanding of how cells respond to various stimuli or treatments.
What are some tips for optimizing Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica assays?
To ensure accurate and reliable results with MTT assays, it is essential to optimize the experimental conditions. Some tips for optimizing MTT assays include standardizing cell culture conditions, choosing the appropriate MTT concentration, and carefully following the protocol provided with the MTT reagent. Additionally, it is important to account for any potential interference from compounds or treatments that may affect the MTT assay results.
Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica is a commonly used compound in biochemistry and cell biology research. It is a yellow tetrazolium salt that is used to measure cell viability and proliferation in cell culture experiments. MTT is converted into a purple formazan product by living cells, allowing researchers to quantify cell growth and metabolic activity.
How is Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica used in research?
MTT is typically used in cell viability assays to assess the effects of various treatments or conditions on cell health. After treating cells with a compound or subjecting them to specific conditions, researchers add MTT to the cell culture medium. Live cells reduce MTT to formazan, which can be quantified using spectrophotometry. The amount of formazan produced is directly proportional to the number of viable cells in the culture.
What are the advantages of using Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica?
One of the main advantages of using MTT in cell viability assays is its simplicity and reliability. The assay can be performed relatively quickly and does not require specialized equipment. Additionally, MTT is non-toxic to cells, allowing for repeated measurements over time. The results obtained with MTT are also highly reproducible, making it a preferred choice for many researchers in the field.
Can Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica be used in combination with other assays?
Yes, MTT can be used in combination with other assays to obtain more comprehensive information about cell viability and proliferation. For example, researchers often perform MTT assays alongside other assays such as lactate dehydrogenase (LDH) release assays to assess cytotoxicity. Combining different assays can provide a more thorough understanding of how cells respond to various stimuli or treatments.
What are some tips for optimizing Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica assays?
To ensure accurate and reliable results with MTT assays, it is essential to optimize the experimental conditions. Some tips for optimizing MTT assays include standardizing cell culture conditions, choosing the appropriate MTT concentration, and carefully following the protocol provided with the MTT reagent. Additionally, it is important to account for any potential interference from compounds or treatments that may affect the MTT assay results.
