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| Name | XTT Sodium Salt BioChemica |
| Type | Biochemical |
| Form | Powder |
| Composition | Sodium salt of XTT (2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide) |
| Purity | ≥ 95% |
| Solubility | Water |
| Storage | Store at room temperature |
| Shelf Life | 2 years |
| Molecular Weight | 625.54 g/mol |
| CAS Number | 111072-31-2 |
| Applications | Cell viability assays, cytotoxicity assays, metabolic activity assays |
FAQ
What is XTT Sodium Salt BioChemica?
BioChemica XTT Sodium Salt is a reagent primarily used in the XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay for assessing cell viability, proliferation, and cytotoxicity. This sodium salt is a colorless to yellowish-white crystalline powder that is soluble in water.
How is BioChemica XTT Sodium Salt used in cell viability assays?
XTT Sodium Salt is reduced by cellular dehydrogenases in living cells to form an orange formazan product that can be measured spectrophotometrically. This conversion is catalyzed by active mitochondrial enzymes in metabolically active cells. The amount of formazan produced is directly proportional to the number of living cells in a sample.
What are the advantages of using XTT Sodium Salt in cell viability assays?
BioChemica XTT Sodium Salt offers several advantages over other viability assays, including high sensitivity, rapid color development, simplicity, and compatibility with a wide range of cell types. The XTT assay does not require the use of radioactivity, tissue culture media, or washing steps, making it a safer and more convenient option for assessing cell viability.
How should XTT Sodium Salt be stored and handled?
XTT Sodium Salt should be stored in a cool, dry place away from light and moisture. It is important to avoid exposure to extreme temperatures and acidic or alkaline conditions, as this can degrade the reagent and affect its performance. When handling XTT Sodium Salt, it is recommended to wear appropriate personal protective equipment, such as gloves and eye protection, to prevent skin and eye contact.
Are there any additional tips for using XTT Sodium Salt in cell viability assays?
It is essential to optimize the XTT assay conditions for each specific cell type and experimental setup to ensure accurate and reliable results. This may involve testing different concentrations of XTT Sodium Salt, incubation times, and the addition of electron-coupling reagents to enhance formazan production. Additionally, proper controls should be included in all experiments to account for variables such as background absorbance and assay precision.
BioChemica XTT Sodium Salt is a reagent primarily used in the XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay for assessing cell viability, proliferation, and cytotoxicity. This sodium salt is a colorless to yellowish-white crystalline powder that is soluble in water.
How is BioChemica XTT Sodium Salt used in cell viability assays?
XTT Sodium Salt is reduced by cellular dehydrogenases in living cells to form an orange formazan product that can be measured spectrophotometrically. This conversion is catalyzed by active mitochondrial enzymes in metabolically active cells. The amount of formazan produced is directly proportional to the number of living cells in a sample.
What are the advantages of using XTT Sodium Salt in cell viability assays?
BioChemica XTT Sodium Salt offers several advantages over other viability assays, including high sensitivity, rapid color development, simplicity, and compatibility with a wide range of cell types. The XTT assay does not require the use of radioactivity, tissue culture media, or washing steps, making it a safer and more convenient option for assessing cell viability.
How should XTT Sodium Salt be stored and handled?
XTT Sodium Salt should be stored in a cool, dry place away from light and moisture. It is important to avoid exposure to extreme temperatures and acidic or alkaline conditions, as this can degrade the reagent and affect its performance. When handling XTT Sodium Salt, it is recommended to wear appropriate personal protective equipment, such as gloves and eye protection, to prevent skin and eye contact.
Are there any additional tips for using XTT Sodium Salt in cell viability assays?
It is essential to optimize the XTT assay conditions for each specific cell type and experimental setup to ensure accurate and reliable results. This may involve testing different concentrations of XTT Sodium Salt, incubation times, and the addition of electron-coupling reagents to enhance formazan production. Additionally, proper controls should be included in all experiments to account for variables such as background absorbance and assay precision.
