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| Name | Bis-Tris for buffer solutions |
| Brand | Thermo Fisher Scientific |
| Type | Buffer solution component |
| Form | Powder |
| Purity | High purity |
| Solubility | Soluble in water |
| Storage | Store at room temperature |
| Packaging | Available in various pack sizes |
| Application | Used in biochemical research |
| PH Range | 5.8 - 7.2 |
| Molecular Weight | 209.25 g/mol |
| Boiling Point | 444.9 °C |
| Melting Point | 156 - 158 °C |
| Density | 1.22 g/cm3 |
| Cas Number | 6976-37-0 |
| Pubchem Cid | 5912296 |
FAQ
What is Bis-Tris and how is it used in buffer solutions?
Bis-Tris is a zwitterionic buffer commonly used in biochemistry and molecular biology applications. It is used to maintain a stable pH in solutions, particularly in the range of 5.8-7.7. Bis-Tris is known for its high buffering capacity and minimal interaction with proteins, making it ideal for biochemical studies.
What are the advantages of using Bis-Tris in buffer solutions?
One of the main advantages of using Bis-Tris in buffer solutions is its ability to maintain a stable pH over a wide range of temperatures. It is also known for its low UV absorbance, making it ideal for spectrophotometric applications. Additionally, Bis-Tris has minimal interference with protein activities, making it a popular choice for protein purification and enzyme assays.
How do you prepare a buffer solution using Bis-Tris?
To prepare a buffer solution using Bis-Tris, you will need to dissolve the appropriate amount of Bis-Tris in water to achieve the desired concentration. Adjust the pH of the solution using a strong acid or base, such as HCl or NaOH, until the desired pH is reached. Be sure to use a pH meter to accurately measure and adjust the pH of the solution.
What are some common applications of Bis-Tris buffer solutions?
Bis-Tris buffer solutions are commonly used in a variety of molecular biology and biochemistry applications. Some common uses include protein purification, enzyme assays, electrophoresis, and cell culture. Bis-Tris is also used in the preparation of PCR buffers and other molecular biology reagents.
Can Bis-Tris buffer solutions be stored long-term?
Yes, Bis-Tris buffer solutions can be stored long-term when properly prepared and stored. It is recommended to aliquot the buffer solution into smaller volumes to avoid frequent freeze-thaw cycles, which can degrade the buffer components. Store the aliquots at -20°C or -80°C for longer-term storage.
Bis-Tris is a zwitterionic buffer commonly used in biochemistry and molecular biology applications. It is used to maintain a stable pH in solutions, particularly in the range of 5.8-7.7. Bis-Tris is known for its high buffering capacity and minimal interaction with proteins, making it ideal for biochemical studies.
What are the advantages of using Bis-Tris in buffer solutions?
One of the main advantages of using Bis-Tris in buffer solutions is its ability to maintain a stable pH over a wide range of temperatures. It is also known for its low UV absorbance, making it ideal for spectrophotometric applications. Additionally, Bis-Tris has minimal interference with protein activities, making it a popular choice for protein purification and enzyme assays.
How do you prepare a buffer solution using Bis-Tris?
To prepare a buffer solution using Bis-Tris, you will need to dissolve the appropriate amount of Bis-Tris in water to achieve the desired concentration. Adjust the pH of the solution using a strong acid or base, such as HCl or NaOH, until the desired pH is reached. Be sure to use a pH meter to accurately measure and adjust the pH of the solution.
What are some common applications of Bis-Tris buffer solutions?
Bis-Tris buffer solutions are commonly used in a variety of molecular biology and biochemistry applications. Some common uses include protein purification, enzyme assays, electrophoresis, and cell culture. Bis-Tris is also used in the preparation of PCR buffers and other molecular biology reagents.
Can Bis-Tris buffer solutions be stored long-term?
Yes, Bis-Tris buffer solutions can be stored long-term when properly prepared and stored. It is recommended to aliquot the buffer solution into smaller volumes to avoid frequent freeze-thaw cycles, which can degrade the buffer components. Store the aliquots at -20°C or -80°C for longer-term storage.
