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| Product Name | HEPES for Cell Culture |
| Supplier | Thermo Fisher Scientific |
| Form | Powder |
| Packaging | Bottle |
| Storage | Room temperature |
| Sterility | Non-sterile |
| Shelf Life | 2 years |
| Concentration | 1 M |
| PH Range | 6.8 - 8.2 |
| Application | Cell culture media |
| Solubility | Soluble in water |
| Appearance | White crystalline powder |
| CAS Number | 7365-45-9 |
| Molecular Weight | 238.3 g/mol |
FAQ
What is HEPES and how is it used in cell culture?
HEPES, or N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, is a buffering agent commonly used in cell culture media. It helps to maintain the pH of the cell culture medium within a stable range, which is crucial for the growth and viability of cells.
Why is HEPES preferred over other buffering agents in cell culture?
HEPES is preferred over other buffering agents in cell culture for several reasons. One of the main advantages of HEPES is its ability to maintain a stable pH in cell culture media without the need for CO2 supplementation. This can be especially beneficial for cells that are sensitive to changes in pH or for experiments that require a tightly controlled environment.
How is HEPES typically used in cell culture experiments?
HEPES is typically added to cell culture media at a concentration of 10-25mM, depending on the specific requirements of the experiment or cell line. It is added to the media before it is sterilized, either by autoclaving or by filtration, to ensure that the pH remains stable throughout the experiment.
Are there any limitations or considerations to keep in mind when using HEPES in cell culture?
While HEPES is a widely used buffering agent in cell culture, there are some limitations and considerations to keep in mind. For example, HEPES can be quite expensive compared to other buffering agents, so cost may be a factor to consider. Additionally, some cells may be sensitive to HEPES or may not grow as well in media containing HEPES, so it is important to optimize the concentration and usage of HEPES for specific cell lines.
What are some best practices for using HEPES in cell culture experiments?
Some best practices for using HEPES in cell culture experiments include storing HEPES powder in a cool, dry place to prevent degradation, using freshly prepared HEPES solutions for each experiment to ensure optimal buffering capacity, and testing the pH of the media before adding cells to verify that the pH is within the desired range. Additionally, it is important to carefully follow the manufacturer's instructions for preparing and using HEPES in cell culture media to achieve the best results.
HEPES, or N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, is a buffering agent commonly used in cell culture media. It helps to maintain the pH of the cell culture medium within a stable range, which is crucial for the growth and viability of cells.
Why is HEPES preferred over other buffering agents in cell culture?
HEPES is preferred over other buffering agents in cell culture for several reasons. One of the main advantages of HEPES is its ability to maintain a stable pH in cell culture media without the need for CO2 supplementation. This can be especially beneficial for cells that are sensitive to changes in pH or for experiments that require a tightly controlled environment.
How is HEPES typically used in cell culture experiments?
HEPES is typically added to cell culture media at a concentration of 10-25mM, depending on the specific requirements of the experiment or cell line. It is added to the media before it is sterilized, either by autoclaving or by filtration, to ensure that the pH remains stable throughout the experiment.
Are there any limitations or considerations to keep in mind when using HEPES in cell culture?
While HEPES is a widely used buffering agent in cell culture, there are some limitations and considerations to keep in mind. For example, HEPES can be quite expensive compared to other buffering agents, so cost may be a factor to consider. Additionally, some cells may be sensitive to HEPES or may not grow as well in media containing HEPES, so it is important to optimize the concentration and usage of HEPES for specific cell lines.
What are some best practices for using HEPES in cell culture experiments?
Some best practices for using HEPES in cell culture experiments include storing HEPES powder in a cool, dry place to prevent degradation, using freshly prepared HEPES solutions for each experiment to ensure optimal buffering capacity, and testing the pH of the media before adding cells to verify that the pH is within the desired range. Additionally, it is important to carefully follow the manufacturer's instructions for preparing and using HEPES in cell culture media to achieve the best results.
