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| Name | Tris Hydrochloride for buffer solutions |
| Chemical Formula | C4H11NO3S |
| Molecular Weight | 149.21 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| PH Range | 7.0 - 9.0 |
| Storage Conditions | Store in a cool, dry place |
| Application | Used as a buffer in biochemical and molecular biology research |
| Purity | Minimum 99% |
| Melting Point | 167-170°C |
| Boiling Point | 160°C at 2mmHg |
| Synonyms | Tris-HCl, Tris Buffer, Tris Hydrochloride |
| Cas Number | 1185-53-1 |
| Pubchem Id | 6395 |
| Chemical Structure | CC(CO)(C(=O)[O-])N.Cl |
FAQ
What is Tris Hydrochloride and how is it used in buffer solutions?
Tris Hydrochloride, also known as Tris HCl, is a chemical compound commonly used in biochemistry and molecular biology as a buffer solution. It helps to maintain a stable pH level in a solution, making it ideal for a variety of laboratory applications.
What are the advantages of using Tris Hydrochloride in buffer solutions?
One of the main advantages of using Tris Hydrochloride in buffer solutions is its ability to effectively maintain a stable pH level over a wide range of temperatures. Additionally, Tris Hydrochloride is known for its compatibility with a variety of biological molecules, making it a versatile choice for many different experiments.
How do I prepare a buffer solution using Tris Hydrochloride?
To prepare a buffer solution using Tris Hydrochloride, simply dissolve the desired amount of Tris HCl in distilled water. Adjust the pH level of the solution as needed with an acid or base, depending on the desired pH level. It is important to accurately measure and mix the components to ensure a successful buffer solution.
Can Tris Hydrochloride be used in combination with other buffer components?
Yes, Tris Hydrochloride can be used in combination with other buffer components to create custom buffer solutions tailored to specific experimental needs. Common additives include EDTA, glycerol, and various salts to further enhance the buffering capacity and stability of the solution.
Are there any considerations or precautions to keep in mind when using Tris Hydrochloride in buffer solutions?
When using Tris Hydrochloride in buffer solutions, it is important to handle the compound with care and follow proper safety protocols. Always wear appropriate personal protective equipment and work in a well-ventilated area when handling Tris Hydrochloride. Additionally, be mindful of the chemical compatibility of Tris Hydrochloride with other reagents or samples being used in the experiment.
Tris Hydrochloride, also known as Tris HCl, is a chemical compound commonly used in biochemistry and molecular biology as a buffer solution. It helps to maintain a stable pH level in a solution, making it ideal for a variety of laboratory applications.
What are the advantages of using Tris Hydrochloride in buffer solutions?
One of the main advantages of using Tris Hydrochloride in buffer solutions is its ability to effectively maintain a stable pH level over a wide range of temperatures. Additionally, Tris Hydrochloride is known for its compatibility with a variety of biological molecules, making it a versatile choice for many different experiments.
How do I prepare a buffer solution using Tris Hydrochloride?
To prepare a buffer solution using Tris Hydrochloride, simply dissolve the desired amount of Tris HCl in distilled water. Adjust the pH level of the solution as needed with an acid or base, depending on the desired pH level. It is important to accurately measure and mix the components to ensure a successful buffer solution.
Can Tris Hydrochloride be used in combination with other buffer components?
Yes, Tris Hydrochloride can be used in combination with other buffer components to create custom buffer solutions tailored to specific experimental needs. Common additives include EDTA, glycerol, and various salts to further enhance the buffering capacity and stability of the solution.
Are there any considerations or precautions to keep in mind when using Tris Hydrochloride in buffer solutions?
When using Tris Hydrochloride in buffer solutions, it is important to handle the compound with care and follow proper safety protocols. Always wear appropriate personal protective equipment and work in a well-ventilated area when handling Tris Hydrochloride. Additionally, be mindful of the chemical compatibility of Tris Hydrochloride with other reagents or samples being used in the experiment.
