D(-)-Sorbitol for molecular biology

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Name D(-)-Sorbitol
Molecular Formula C6H14O6
Molecular Weight 182.17 g/mol
Cas Number 50-70-4
Appearance White crystalline powder
Solubility Soluble in water
Storage Conditions Store at room temperature
Usage Commonly used as a cryoprotectant in molecular biology applications
Purity ≥ 98%
Specific Rotations -8 to -18° (c=10%, H2O, 20°C)
Melting Point 95-105°C
Boiling Point 271-273°C
Density 1.49 g/cm3
Flash Point 176°C
Risk Phrases R36/37/38 - Irritating to eyes, respiratory system, and skin
Safety Phrases S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
Manufacturing Source Chemical synthesis
FAQ
What is D(-) Sorbitol and how is it used in molecular biology?
D(-) Sorbitol, also known as sorbitol or glucitol, is a sugar alcohol that is commonly used in molecular biology as a cryoprotectant and as a reagent for the isolation of nucleic acids and proteins. It is a versatile compound that can be used in a variety of applications due to its unique properties.

How does D(-) Sorbitol function as a cryoprotectant?
D(-) Sorbitol functions as a cryoprotectant by reducing the formation of ice crystals during the freezing process. It helps to protect cells and tissues from damage caused by freezing and thawing by acting as a chemical protectant and stabilizer. This makes it an essential component in the preservation of biological samples for long-term storage.

What are the benefits of using D(-) Sorbitol in the isolation of nucleic acids and proteins?
D(-) Sorbitol is an effective reagent for the isolation of nucleic acids and proteins due to its ability to disrupt protein interactions and facilitate the precipitation and purification of biomolecules. It helps to improve the yield and purity of isolated nucleic acids and proteins, making it an ideal choice for research laboratories and biotechnology companies.

Can D(-) Sorbitol be used in combination with other reagents in molecular biology experiments?
Yes, D(-) Sorbitol can be used in combination with other reagents in molecular biology experiments to enhance their performance and efficiency. It can be used in conjunction with various buffers, enzymes, and chemicals to optimize experimental conditions and achieve reliable results. Its compatibility with a wide range of reagents makes it a valuable tool for researchers working in the field of molecular biology.

Are there any precautions or storage recommendations when using D(-) Sorbitol in molecular biology experiments?
It is important to store D(-) Sorbitol in a cool, dry place away from direct sunlight to prevent degradation and contamination. Additionally, proper handling procedures should be followed to ensure the safety of laboratory personnel and the integrity of experimental samples. Precautions should be taken to avoid contact with skin, eyes, and mucous membranes, and appropriate personal protective equipment should be worn when handling D(-) Sorbitol in the laboratory.
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