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| Name | HEPES for buffer solutions |
| Brand | Thermo Fisher Scientific |
| Product Type | Chemical |
| Quantity | 500g |
| Purity | ≥99% |
| Form | Powder |
| Solubility | Soluble in water |
| Storage Condition | Store at room temperature |
| Usage | Used in biological research as a buffering agent |
| Molecular Weight | 238.3 g/mol |
| Melting Point | 234-236°C |
FAQ
What is HEPES and why is it used for buffer solutions?
HEPES, or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a zwitterionic organic chemical compound that is commonly used in biological and biochemical research as a buffering agent. It is especially useful for buffering cell culture media due to its strong buffering capacity and minimal impact on cell physiology.
What are the advantages of using HEPES for buffer solutions?
HEPES is ideal for maintaining a stable pH in cell culture media, as it has a pKa value of 7.55 at 25°C, which is very close to the physiological pH of 7.4. This property allows HEPES to effectively buffer solutions in the physiological range, making it a popular choice for cell culture applications.
Can HEPES be used in a wide range of experimental conditions?
Yes, HEPES is highly versatile and can be used in a wide range of experimental conditions. It is compatible with both aqueous and organic solvents, and is stable over a wide pH range (6.8-8.2). Additionally, HEPES has low UV absorbance, making it suitable for use in spectroscopic applications.
How should HEPES be stored to maintain its stability?
HEPES should be stored at room temperature in a dry and dark place to maintain its stability. It is important to keep HEPES away from moisture, as it is hygroscopic and can absorb water from the atmosphere. Proper storage will ensure that HEPES remains effective as a buffering agent.
Are there any precautions to consider when working with HEPES solutions?
While HEPES is generally considered safe to use, it is important to handle it with care and avoid ingestion or contact with skin and eyes. It is also recommended to wear appropriate personal protective equipment, such as gloves and goggles, when handling HEPES solutions. Additionally, HEPES should be disposed of properly according to local regulations to prevent environmental contamination.
HEPES, or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, is a zwitterionic organic chemical compound that is commonly used in biological and biochemical research as a buffering agent. It is especially useful for buffering cell culture media due to its strong buffering capacity and minimal impact on cell physiology.
What are the advantages of using HEPES for buffer solutions?
HEPES is ideal for maintaining a stable pH in cell culture media, as it has a pKa value of 7.55 at 25°C, which is very close to the physiological pH of 7.4. This property allows HEPES to effectively buffer solutions in the physiological range, making it a popular choice for cell culture applications.
Can HEPES be used in a wide range of experimental conditions?
Yes, HEPES is highly versatile and can be used in a wide range of experimental conditions. It is compatible with both aqueous and organic solvents, and is stable over a wide pH range (6.8-8.2). Additionally, HEPES has low UV absorbance, making it suitable for use in spectroscopic applications.
How should HEPES be stored to maintain its stability?
HEPES should be stored at room temperature in a dry and dark place to maintain its stability. It is important to keep HEPES away from moisture, as it is hygroscopic and can absorb water from the atmosphere. Proper storage will ensure that HEPES remains effective as a buffering agent.
Are there any precautions to consider when working with HEPES solutions?
While HEPES is generally considered safe to use, it is important to handle it with care and avoid ingestion or contact with skin and eyes. It is also recommended to wear appropriate personal protective equipment, such as gloves and goggles, when handling HEPES solutions. Additionally, HEPES should be disposed of properly according to local regulations to prevent environmental contamination.
