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| Name | PMSF BioChemica |
| Supplier | MilliporeSigma |
| Product Type | Protease Inhibitor |
| CAS Number | 329-98-6 |
| Appearance | White crystalline powder |
| Solubility | Soluble in ethanol, acetone, dimethyl sulfoxide |
| Stability | Stable under recommended storage conditions |
| Storage Temperature | 2-8°C |
| Shelf Life | Minimum of 2 years |
| Usage | Used for inhibiting serine proteases in biological samples |
| Applications | Protein purification, enzyme assays, cell lysis |
FAQ
What is PMSF BioChemica and what are its primary uses?
PMSF BioChemica is a potent serine protease inhibitor that is widely used in biochemistry and molecular biology research. It is commonly used to inhibit serine proteases in biological samples, thus preventing the degradation of proteins. This compound is essential for preserving protein integrity and stability in various laboratory experiments.
How does PMSF BioChemica work and what are its mechanisms of action?
PMSF BioChemica works by irreversibly binding to the active site of serine proteases, thereby inhibiting their enzymatic activity. This compound forms a covalent bond with the serine residue in the protease's active site, leading to the inactivation of the enzyme. By inhibiting serine proteases, PMSF BioChemica effectively prevents the degradation of proteins and helps maintain their structural integrity.
What are the advantages of using PMSF BioChemica in research applications?
PMSF BioChemica offers several advantages in research applications, including its high potency and specificity for serine proteases. This compound is known for its rapid inhibition of protease activity, making it ideal for time-sensitive experiments. Additionally, PMSF BioChemica is stable in solution and easy to use, making it a popular choice among researchers for protein protection and preservation.
How should PMSF BioChemica be handled and stored to ensure its effectiveness?
PMSF BioChemica should be handled with care due to its toxicity and potential hazards. It is important to wear appropriate personal protective equipment, such as gloves and goggles, when working with this compound. PMSF BioChemica should be stored in a cool, dry place away from direct sunlight and moisture to prevent degradation. It is also crucial to follow proper disposal procedures for PMSF BioChemica to minimize environmental impact.
What are some common applications of PMSF BioChemica in biochemical and molecular biology research?
PMSF BioChemica is commonly used in various biochemical and molecular biology research applications, including protein purification, enzyme assays, and western blotting. This compound is essential for inhibiting serine proteases during protein extraction and purification processes to prevent protein degradation. Additionally, PMSF BioChemica is used in enzyme assays to study the activity of serine proteases and other enzymes. In western blotting, PMSF BioChemica is often included in lysis buffers to preserve protein integrity during sample preparation.
PMSF BioChemica is a potent serine protease inhibitor that is widely used in biochemistry and molecular biology research. It is commonly used to inhibit serine proteases in biological samples, thus preventing the degradation of proteins. This compound is essential for preserving protein integrity and stability in various laboratory experiments.
How does PMSF BioChemica work and what are its mechanisms of action?
PMSF BioChemica works by irreversibly binding to the active site of serine proteases, thereby inhibiting their enzymatic activity. This compound forms a covalent bond with the serine residue in the protease's active site, leading to the inactivation of the enzyme. By inhibiting serine proteases, PMSF BioChemica effectively prevents the degradation of proteins and helps maintain their structural integrity.
What are the advantages of using PMSF BioChemica in research applications?
PMSF BioChemica offers several advantages in research applications, including its high potency and specificity for serine proteases. This compound is known for its rapid inhibition of protease activity, making it ideal for time-sensitive experiments. Additionally, PMSF BioChemica is stable in solution and easy to use, making it a popular choice among researchers for protein protection and preservation.
How should PMSF BioChemica be handled and stored to ensure its effectiveness?
PMSF BioChemica should be handled with care due to its toxicity and potential hazards. It is important to wear appropriate personal protective equipment, such as gloves and goggles, when working with this compound. PMSF BioChemica should be stored in a cool, dry place away from direct sunlight and moisture to prevent degradation. It is also crucial to follow proper disposal procedures for PMSF BioChemica to minimize environmental impact.
What are some common applications of PMSF BioChemica in biochemical and molecular biology research?
PMSF BioChemica is commonly used in various biochemical and molecular biology research applications, including protein purification, enzyme assays, and western blotting. This compound is essential for inhibiting serine proteases during protein extraction and purification processes to prevent protein degradation. Additionally, PMSF BioChemica is used in enzyme assays to study the activity of serine proteases and other enzymes. In western blotting, PMSF BioChemica is often included in lysis buffers to preserve protein integrity during sample preparation.
