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| Name | Proteinase K, recombinant |
| Type | Enzyme |
| Source | Recombinant |
| Species | None |
| Activity | Broad-spectrum serine protease |
| Substrate | Peptide bonds |
| Optimal PH | 7.5-9.5 |
| Optimal Temperature | 37-65°C |
| Applications | DNA and RNA extraction, protein purification, tissue dissociation |
| Storage | Store at -20°C |
| Stability | Stable at room temperature for short periods |
| Appearance | White to off-white powder |
| Solubility | Soluble in water |
FAQ
What is Proteinase K?
Proteinase K is a recombinant enzyme that belongs to the serine protease family. It is widely used in molecular biology applications to digest proteins in samples such as tissues, cells, and nucleic acids.
How does Proteinase K work?
Proteinase K works by cleaving peptide bonds in proteins, resulting in the degradation of the protein molecules. It is highly effective at breaking down a wide range of proteins, making it a valuable tool in various research applications.
What are the key features of Proteinase K?
Proteinase K exhibits high specificity and activity at a broad range of temperatures and pH levels, making it a versatile enzyme for protein digestion. It is also stable and active in the presence of denaturing agents like SDS and urea, making it suitable for use in various experimental conditions.
How is Proteinase K used in research?
Proteinase K is commonly used in molecular biology research for tasks such as DNA and RNA extraction, protein purification, and cell lysis. It can effectively remove proteins that may interfere with downstream applications like PCR, sequencing, and protein analysis.
What are the benefits of using Proteinase K?
Proteinase K offers researchers a reliable and efficient method for protein digestion in various biological samples. Its high activity, stability, and specificity make it an essential tool for a wide range of molecular biology experiments, enabling researchers to obtain accurate and reliable results.
Proteinase K is a recombinant enzyme that belongs to the serine protease family. It is widely used in molecular biology applications to digest proteins in samples such as tissues, cells, and nucleic acids.
How does Proteinase K work?
Proteinase K works by cleaving peptide bonds in proteins, resulting in the degradation of the protein molecules. It is highly effective at breaking down a wide range of proteins, making it a valuable tool in various research applications.
What are the key features of Proteinase K?
Proteinase K exhibits high specificity and activity at a broad range of temperatures and pH levels, making it a versatile enzyme for protein digestion. It is also stable and active in the presence of denaturing agents like SDS and urea, making it suitable for use in various experimental conditions.
How is Proteinase K used in research?
Proteinase K is commonly used in molecular biology research for tasks such as DNA and RNA extraction, protein purification, and cell lysis. It can effectively remove proteins that may interfere with downstream applications like PCR, sequencing, and protein analysis.
What are the benefits of using Proteinase K?
Proteinase K offers researchers a reliable and efficient method for protein digestion in various biological samples. Its high activity, stability, and specificity make it an essential tool for a wide range of molecular biology experiments, enabling researchers to obtain accurate and reliable results.
